Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24326 | 5' | -56.7 | NC_005263.2 | + | 27317 | 0.69 | 0.45308 |
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Target: 5'- aCGGCGCGAcccgACGcGCCGA---CGAUCa -3' miRNA: 3'- -GCCGCGCU----UGCaCGGCUagcGCUAGa -5' |
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| 24326 | 5' | -56.7 | NC_005263.2 | + | 6254 | 0.69 | 0.433748 |
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Target: 5'- aGGCGCGGuCGUGCCG-UCGgGcagCUu -3' miRNA: 3'- gCCGCGCUuGCACGGCuAGCgCua-GA- -5' |
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| 24326 | 5' | -56.7 | NC_005263.2 | + | 9768 | 0.7 | 0.39657 |
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Target: 5'- cCGGuCGCc-GCGUGCaCGGUCGCGcgCg -3' miRNA: 3'- -GCC-GCGcuUGCACG-GCUAGCGCuaGa -5' |
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| 24326 | 5' | -56.7 | NC_005263.2 | + | 1583 | 0.7 | 0.39657 |
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Target: 5'- aCGGCacGCuuGGACGUGCCGAgcagCGCGGc-- -3' miRNA: 3'- -GCCG--CG--CUUGCACGGCUa---GCGCUaga -5' |
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| 24326 | 5' | -56.7 | NC_005263.2 | + | 20271 | 0.7 | 0.392069 |
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Target: 5'- gCGGCGcCGAauucaacGCGUGCCGcgaaggugacgcCGCGAUCa -3' miRNA: 3'- -GCCGC-GCU-------UGCACGGCua----------GCGCUAGa -5' |
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| 24326 | 5' | -56.7 | NC_005263.2 | + | 14193 | 0.7 | 0.387601 |
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Target: 5'- gGGCagcaagcguuccGCGAcgGCGUGCCGuUCGCGcgCa -3' miRNA: 3'- gCCG------------CGCU--UGCACGGCuAGCGCuaGa -5' |
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| 24326 | 5' | -56.7 | NC_005263.2 | + | 6508 | 0.7 | 0.370069 |
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Target: 5'- aCGGCGCGuugaaGCCGGUCGUGAg-- -3' miRNA: 3'- -GCCGCGCuugcaCGGCUAGCGCUaga -5' |
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| 24326 | 5' | -56.7 | NC_005263.2 | + | 7022 | 0.77 | 0.151049 |
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Target: 5'- gCGGCGCGaAACGUGUCGAcgaUCGUGcgCg -3' miRNA: 3'- -GCCGCGC-UUGCACGGCU---AGCGCuaGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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