Results 61 - 62 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24327 | 5' | -54.3 | NC_005263.2 | + | 17710 | 0.66 | 0.786667 |
Target: 5'- aGCCGGUCGcGCUAuccgaaGCGAUCGCg- -3' miRNA: 3'- -CGGCCAGC-UGGUcaag--UGCUAGUGgc -5' |
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24327 | 5' | -54.3 | NC_005263.2 | + | 26533 | 0.66 | 0.786667 |
Target: 5'- cGCCGG-CGGCgAGcaCGCGG-CGCUGg -3' miRNA: 3'- -CGGCCaGCUGgUCaaGUGCUaGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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