Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24333 | 5' | -50.6 | NC_005264.1 | + | 136563 | 0.66 | 0.990977 |
Target: 5'- --cGAUC-GCGAuCCAGGAacgCGCCGc- -3' miRNA: 3'- aaaUUAGaCGCU-GGUCCUa--GCGGUau -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 85071 | 0.66 | 0.988033 |
Target: 5'- ----cUCUGCGGCCGcgucgucagauucGGAUacggCGCCGUAg -3' miRNA: 3'- aaauuAGACGCUGGU-------------CCUA----GCGGUAU- -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 109503 | 0.66 | 0.986564 |
Target: 5'- --cAGUCUGUaGCCAGGuaCGCCGUu -3' miRNA: 3'- aaaUUAGACGcUGGUCCuaGCGGUAu -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 101062 | 0.68 | 0.966461 |
Target: 5'- -----gCUGCucgagucGAUCAGGGUCGCCAa- -3' miRNA: 3'- aaauuaGACG-------CUGGUCCUAGCGGUau -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 126225 | 0.69 | 0.937377 |
Target: 5'- --cAAUCUGUGAUCGGGAgCGCuCAa- -3' miRNA: 3'- aaaUUAGACGCUGGUCCUaGCG-GUau -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 124033 | 0.71 | 0.901902 |
Target: 5'- ---cGUCcGCG-CCGGGGUCGCCGc- -3' miRNA: 3'- aaauUAGaCGCuGGUCCUAGCGGUau -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 5006 | 0.71 | 0.901902 |
Target: 5'- ---cGUCcGCG-CCGGGGUCGCCGc- -3' miRNA: 3'- aaauUAGaCGCuGGUCCUAGCGGUau -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 161506 | 0.71 | 0.883587 |
Target: 5'- --aGGUCUGCGugucCCcgcucgcuggguuuaGGGAUCGCCGUAc -3' miRNA: 3'- aaaUUAGACGCu---GG---------------UCCUAGCGGUAU- -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 42479 | 0.71 | 0.883587 |
Target: 5'- --aGGUCUGCGugucCCcgcucgcuggguuuaGGGAUCGCCGUAc -3' miRNA: 3'- aaaUUAGACGCu---GG---------------UCCUAGCGGUAU- -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 27633 | 0.73 | 0.822611 |
Target: 5'- ---cAUCUGgGucucaGCCAGGAUCGCCGa- -3' miRNA: 3'- aaauUAGACgC-----UGGUCCUAGCGGUau -5' |
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24333 | 5' | -50.6 | NC_005264.1 | + | 161098 | 1.05 | 0.013707 |
Target: 5'- uUUUAAUCUGCGACCAGGAUCGCCAUAg -3' miRNA: 3'- -AAAUUAGACGCUGGUCCUAGCGGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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