Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24334 | 3' | -53.4 | NC_005264.1 | + | 18094 | 0.66 | 0.953732 |
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Target: 5'- cUGCUGUuaUGCCGgcagaUGGC-GCGAGa -3' miRNA: 3'- cACGACGuaACGGUag---ACCGaCGUUC- -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 108528 | 0.66 | 0.953732 |
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Target: 5'- cUGCUGgAUcgUGCCGagaaUCUGG-UGCAGGu -3' miRNA: 3'- cACGACgUA--ACGGU----AGACCgACGUUC- -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 75214 | 0.66 | 0.949488 |
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Target: 5'- cGUGCagagGguUUGCC-UCUGGCgcgGCcAGg -3' miRNA: 3'- -CACGa---CguAACGGuAGACCGa--CGuUC- -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 135389 | 0.66 | 0.944992 |
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Target: 5'- uUGCUGCGccGCUcggcgcagGUCUGGCUGaAAGc -3' miRNA: 3'- cACGACGUaaCGG--------UAGACCGACgUUC- -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 18133 | 0.66 | 0.940242 |
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Target: 5'- cGUGCU----UGCUGUCUGGC-GCGAGc -3' miRNA: 3'- -CACGAcguaACGGUAGACCGaCGUUC- -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 47284 | 0.67 | 0.935235 |
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Target: 5'- -gGCUGUgguguggagAUUGCCAUgaUGGCaGCGGGg -3' miRNA: 3'- caCGACG---------UAACGGUAg-ACCGaCGUUC- -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 125918 | 0.69 | 0.830045 |
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Target: 5'- cGUGUUGCuGUUGCC--UUGGCUGCu-- -3' miRNA: 3'- -CACGACG-UAACGGuaGACCGACGuuc -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 6892 | 0.69 | 0.830045 |
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Target: 5'- cGUGUUGCuGUUGCC--UUGGCUGCu-- -3' miRNA: 3'- -CACGACG-UAACGGuaGACCGACGuuc -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 50028 | 0.7 | 0.803025 |
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Target: 5'- aUGCUGCcgUGCC-UCgaGGUUGCGGc -3' miRNA: 3'- cACGACGuaACGGuAGa-CCGACGUUc -5' |
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| 24334 | 3' | -53.4 | NC_005264.1 | + | 160133 | 1.08 | 0.004393 |
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Target: 5'- aGUGCUGCAUUGCCAUCUGGCUGCAAGc -3' miRNA: 3'- -CACGACGUAACGGUAGACCGACGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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