Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24334 | 5' | -56.5 | NC_005264.1 | + | 115964 | 0.66 | 0.91033 |
Target: 5'- gGUacGCGGcGCCCGACaGGGACGc-- -3' miRNA: 3'- gCGaaCGCC-UGGGCUGaUCCUGCagu -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 100281 | 0.66 | 0.91033 |
Target: 5'- cCGCgcaGCGGGCUCGGCUugcuggaaccGGGAUcUCGg -3' miRNA: 3'- -GCGaa-CGCCUGGGCUGA----------UCCUGcAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 85449 | 0.66 | 0.906122 |
Target: 5'- gCGCUUGCcGGCgucugggCCGACUccagugagcuuugccAGGAUGUCGc -3' miRNA: 3'- -GCGAACGcCUG-------GGCUGA---------------UCCUGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 52416 | 0.66 | 0.904284 |
Target: 5'- uGCUUGUagauugcguuGGGCCCGACgcuuuugAGcaGCGUCAg -3' miRNA: 3'- gCGAACG----------CCUGGGCUGa------UCc-UGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 97921 | 0.66 | 0.900547 |
Target: 5'- uCGCUuucuuccacaucgaaUGCGGG-CUGGCgcaucuGGACGUCAa -3' miRNA: 3'- -GCGA---------------ACGCCUgGGCUGau----CCUGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 84963 | 0.66 | 0.898011 |
Target: 5'- uCGCUUGCGG-CCaGGCUGcuGCGUUAg -3' miRNA: 3'- -GCGAACGCCuGGgCUGAUccUGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 21221 | 0.66 | 0.891513 |
Target: 5'- gGCggUGCcccGACCgCGAUUuGGACGUCAu -3' miRNA: 3'- gCGa-ACGc--CUGG-GCUGAuCCUGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 25501 | 0.67 | 0.877857 |
Target: 5'- gGCUagGauGGCCgCGuCUAGGAUGUCAa -3' miRNA: 3'- gCGAa-CgcCUGG-GCuGAUCCUGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 44879 | 0.67 | 0.863349 |
Target: 5'- aGCcuggggagGCGGGCCCGucgucguCUGGGGCGg-- -3' miRNA: 3'- gCGaa------CGCCUGGGCu------GAUCCUGCagu -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 58005 | 0.67 | 0.848032 |
Target: 5'- gGCUcaGCGGcCCCGGCggacAGGcCGUCGu -3' miRNA: 3'- gCGAa-CGCCuGGGCUGa---UCCuGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 50583 | 0.68 | 0.840084 |
Target: 5'- gCGCacGCGGcgcugagcaGCCCGGCgcuGGACGUUc -3' miRNA: 3'- -GCGaaCGCC---------UGGGCUGau-CCUGCAGu -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 154370 | 0.68 | 0.823646 |
Target: 5'- uGCUUGgGGACCCaGCUGgcGGAUgcuaugGUCAg -3' miRNA: 3'- gCGAACgCCUGGGcUGAU--CCUG------CAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 9058 | 0.69 | 0.751764 |
Target: 5'- aGCgUUGaGGACCCGACgcgcugAGGACGa-- -3' miRNA: 3'- gCG-AACgCCUGGGCUGa-----UCCUGCagu -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 128085 | 0.69 | 0.751764 |
Target: 5'- aGCgUUGaGGACCCGACgcgcugAGGACGa-- -3' miRNA: 3'- gCG-AACgCCUGGGCUGa-----UCCUGCagu -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 4831 | 0.69 | 0.751764 |
Target: 5'- uGCacGCGGGCCUcuguuuCUAGGGCGUUAg -3' miRNA: 3'- gCGaaCGCCUGGGcu----GAUCCUGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 131469 | 0.7 | 0.692324 |
Target: 5'- gCGCggUGCGGucuaaagGCCCGGC-AGG-CGUCAg -3' miRNA: 3'- -GCGa-ACGCC-------UGGGCUGaUCCuGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 12442 | 0.7 | 0.692324 |
Target: 5'- gCGCggUGCGGucuaaagGCCCGGC-AGG-CGUCAg -3' miRNA: 3'- -GCGa-ACGCC-------UGGGCUGaUCCuGCAGU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 54411 | 0.71 | 0.653242 |
Target: 5'- uCGUUguugcGCGGcGCgCCGACUAGGGCGUgGg -3' miRNA: 3'- -GCGAa----CGCC-UG-GGCUGAUCCUGCAgU- -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 119488 | 0.71 | 0.643162 |
Target: 5'- uGCUUGUGGAUgUGGCUgcgcAGGGCGUg- -3' miRNA: 3'- gCGAACGCCUGgGCUGA----UCCUGCAgu -5' |
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24334 | 5' | -56.5 | NC_005264.1 | + | 20293 | 0.72 | 0.592786 |
Target: 5'- gCGCUUGCGGcCCCGA--GGGGCugagGUCGc -3' miRNA: 3'- -GCGAACGCCuGGGCUgaUCCUG----CAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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