Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24335 | 5' | -58.5 | NC_005264.1 | + | 153878 | 0.68 | 0.698801 |
|
Target: 5'- uUUUGGUGCUGcagcGAguugGCCgGCCCgaagGCCGAg -3' miRNA: 3'- -AGACCGCGAC----UUa---CGGgUGGG----UGGCU- -5' |
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| 24335 | 5' | -58.5 | NC_005264.1 | + | 156297 | 0.69 | 0.668917 |
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Target: 5'- --aGGCGCUccugccGAAgccCCCugCCGCCGAu -3' miRNA: 3'- agaCCGCGA------CUUac-GGGugGGUGGCU- -5' |
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| 24335 | 5' | -58.5 | NC_005264.1 | + | 159567 | 1.09 | 0.001729 |
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Target: 5'- cUCUGGCGCUGAAUGCCCACCCACCGAg -3' miRNA: 3'- -AGACCGCGACUUACGGGUGGGUGGCU- -5' |
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| 24335 | 5' | -58.5 | NC_005264.1 | + | 159835 | 0.69 | 0.618638 |
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Target: 5'- --gGGCGCUGGAUGauuagCGCCgGCCGGu -3' miRNA: 3'- agaCCGCGACUUACgg---GUGGgUGGCU- -5' |
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| 24335 | 5' | -58.5 | NC_005264.1 | + | 160338 | 0.67 | 0.737846 |
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Target: 5'- ---cGCGCUGAAgucgcgcgagGCCCGCCgACCa- -3' miRNA: 3'- agacCGCGACUUa---------CGGGUGGgUGGcu -5' |
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| 24335 | 5' | -58.5 | NC_005264.1 | + | 161213 | 0.71 | 0.500488 |
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Target: 5'- -aUGGCagGUUGGA-GCCgCGCCCACCGGc -3' miRNA: 3'- agACCG--CGACUUaCGG-GUGGGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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