Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24337 | 5' | -54.7 | NC_005264.1 | + | 113853 | 0.67 | 0.9084 |
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Target: 5'- aUCAcGGUGGauUGCGAGGAAgaguucugcGCCUacUGCu -3' miRNA: 3'- cAGU-CCACC--ACGUUCCUU---------CGGA--ACGc -5' |
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| 24337 | 5' | -54.7 | NC_005264.1 | + | 162176 | 0.68 | 0.839899 |
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Target: 5'- -aCGGGUGGaGCGGGGAggggggggcgcgugAGCCUcuaucgcgcugccUGCGa -3' miRNA: 3'- caGUCCACCaCGUUCCU--------------UCGGA-------------ACGC- -5' |
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| 24337 | 5' | -54.7 | NC_005264.1 | + | 43149 | 0.68 | 0.839899 |
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Target: 5'- -aCGGGUGGaGCGGGGAggggggggcgcgugAGCCUcuaucgcgcugccUGCGa -3' miRNA: 3'- caGUCCACCaCGUUCCU--------------UCGGA-------------ACGC- -5' |
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| 24337 | 5' | -54.7 | NC_005264.1 | + | 35734 | 0.69 | 0.817678 |
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Target: 5'- aGUCAGGaUGGUGCcggcGAGGAGuaccgguuGCCUaaggaagugguUGCGa -3' miRNA: 3'- -CAGUCC-ACCACG----UUCCUU--------CGGA-----------ACGC- -5' |
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| 24337 | 5' | -54.7 | NC_005264.1 | + | 158839 | 0.69 | 0.80881 |
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Target: 5'- -cCuGGUGG-GCGAGGAGGCaugUGCc -3' miRNA: 3'- caGuCCACCaCGUUCCUUCGga-ACGc -5' |
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| 24337 | 5' | -54.7 | NC_005264.1 | + | 39813 | 0.69 | 0.80881 |
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Target: 5'- -cCuGGUGG-GCGAGGAGGCaugUGCc -3' miRNA: 3'- caGuCCACCaCGUUCCUUCGga-ACGc -5' |
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| 24337 | 5' | -54.7 | NC_005264.1 | + | 106732 | 0.72 | 0.629928 |
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Target: 5'- gGUCgacaAGGaUGGUGCGGGGGAGCCgccUGUu -3' miRNA: 3'- -CAG----UCC-ACCACGUUCCUUCGGa--ACGc -5' |
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| 24337 | 5' | -54.7 | NC_005264.1 | + | 20245 | 0.74 | 0.557536 |
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Target: 5'- -aCGGGUGG-GCAGGGGuugcggcgcGGCCUcGCGg -3' miRNA: 3'- caGUCCACCaCGUUCCU---------UCGGAaCGC- -5' |
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| 24337 | 5' | -54.7 | NC_005264.1 | + | 159068 | 1.09 | 0.003655 |
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Target: 5'- cGUCAGGUGGUGCAAGGAAGCCUUGCGu -3' miRNA: 3'- -CAGUCCACCACGUUCCUUCGGAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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