miRNA: 3'- -CAUCGGGUC-GUCCCGca-GGA---CGa--GCG- -5'
miRNA: 3'- caUCGGG--UCGUC-CCgCagGACGAGCg -5'
Results 61 - 62 of 62 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24338 | 5' | -60.4 | NC_005264.1 | + | 42251 | 0.67 | 0.659442 |
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Target: 5'- cGUAGUCCGGcCGGGGCccgCCUauuGCcggCGCg -3' miRNA: 3'- -CAUCGGGUC-GUCCCGca-GGA---CGa--GCG- -5' |
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| 24338 | 5' | -60.4 | NC_005264.1 | + | 149576 | 0.66 | 0.728213 |
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Target: 5'- -cAGCCCcgGGCGGaGGgGggCUGCUCGa -3' miRNA: 3'- caUCGGG--UCGUC-CCgCagGACGAGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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