Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24339 | 3' | -56.5 | NC_005264.1 | + | 39632 | 0.66 | 0.918065 |
Target: 5'- aCGGcGCUGUGGUUuugccgcGCGgCCUCGu -3' miRNA: 3'- aGCC-CGGCACCAAucuau--CGC-GGAGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 34155 | 0.66 | 0.918065 |
Target: 5'- uUCGGGUCGUGGUacc---GCGCCc-- -3' miRNA: 3'- -AGCCCGGCACCAaucuauCGCGGagc -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 10885 | 0.66 | 0.906324 |
Target: 5'- -gGGGCCGUGG--GGAauucccGGUGCC-CGg -3' miRNA: 3'- agCCCGGCACCaaUCUa-----UCGCGGaGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 129911 | 0.66 | 0.906324 |
Target: 5'- -gGGGCCGUGG--GGAauucccGGUGCC-CGg -3' miRNA: 3'- agCCCGGCACCaaUCUa-----UCGCGGaGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 124163 | 0.66 | 0.900109 |
Target: 5'- cUGGGCaaCGUGGUgaug-GGCGCCgccgCGg -3' miRNA: 3'- aGCCCG--GCACCAaucuaUCGCGGa---GC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 97266 | 0.66 | 0.899474 |
Target: 5'- cCGGGCCuUGGcacGGUAGUggccgcuGCCUCGc -3' miRNA: 3'- aGCCCGGcACCaauCUAUCG-------CGGAGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 33371 | 0.67 | 0.880114 |
Target: 5'- cUCGaGUCG-GGUUuGGUAGCGCUUUGg -3' miRNA: 3'- -AGCcCGGCaCCAAuCUAUCGCGGAGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 113692 | 0.67 | 0.87229 |
Target: 5'- gUCGGGgUGcaaggcuUGGgcAGA-GGCGCCUCGc -3' miRNA: 3'- -AGCCCgGC-------ACCaaUCUaUCGCGGAGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 43203 | 0.67 | 0.858181 |
Target: 5'- gCGGGCCGgcggGGggGGggGGgGCUaCGg -3' miRNA: 3'- aGCCCGGCa---CCaaUCuaUCgCGGaGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 13815 | 0.67 | 0.858181 |
Target: 5'- -gGGGCUGUGGgac-GUGGCGUCUa- -3' miRNA: 3'- agCCCGGCACCaaucUAUCGCGGAgc -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 162230 | 0.67 | 0.858181 |
Target: 5'- gCGGGCCGgcggGGggGGggGGgGCUaCGg -3' miRNA: 3'- aGCCCGGCa---CCaaUCuaUCgCGGaGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 35795 | 0.67 | 0.850463 |
Target: 5'- gUGGcGCCGUcg-UGGcgGGCGCCUCu -3' miRNA: 3'- aGCC-CGGCAccaAUCuaUCGCGGAGc -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 154821 | 0.67 | 0.850463 |
Target: 5'- gUGGcGCCGUcg-UGGcgGGCGCCUCu -3' miRNA: 3'- aGCC-CGGCAccaAUCuaUCGCGGAGc -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 81861 | 0.69 | 0.782354 |
Target: 5'- gUCGGcGUCGaggaagaaauUGGUc-GAUGGCGCCUUGg -3' miRNA: 3'- -AGCC-CGGC----------ACCAauCUAUCGCGGAGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 47400 | 0.69 | 0.754414 |
Target: 5'- -aGuGGCgCGUGGUUGGGU-GCGCCg-- -3' miRNA: 3'- agC-CCG-GCACCAAUCUAuCGCGGagc -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 40071 | 0.69 | 0.744878 |
Target: 5'- gCGGGCC-UGGUacccgAGCGCUUCGu -3' miRNA: 3'- aGCCCGGcACCAaucuaUCGCGGAGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 1216 | 0.71 | 0.665863 |
Target: 5'- aUGGGCCGUGGggc----GUGCCUCa -3' miRNA: 3'- aGCCCGGCACCaaucuauCGCGGAGc -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 52203 | 0.71 | 0.665863 |
Target: 5'- -aGGGCCGUGGggcgcGGUGGCgcgagcugguacGCCUUGa -3' miRNA: 3'- agCCCGGCACCaau--CUAUCG------------CGGAGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 80229 | 0.72 | 0.585086 |
Target: 5'- cUUGGGCCGUGGacggAGAUAaugggcaagucuGUGCUUCGu -3' miRNA: 3'- -AGCCCGGCACCaa--UCUAU------------CGCGGAGC- -5' |
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24339 | 3' | -56.5 | NC_005264.1 | + | 156912 | 1.08 | 0.003212 |
Target: 5'- uUCGGGCCGUGGUUAGAUAGCGCCUCGc -3' miRNA: 3'- -AGCCCGGCACCAAUCUAUCGCGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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