Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24339 | 5' | -51.8 | NC_005264.1 | + | 150741 | 0.66 | 0.991969 |
Target: 5'- cGCgCGGGGCGUACUuguucGCgUCGugUAa-- -3' miRNA: 3'- -CG-GCUCCGCAUGA-----UGgAGUugAUgac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 31715 | 0.66 | 0.991969 |
Target: 5'- cGCgCGGGGCGUACUuguucGCgUCGugUAa-- -3' miRNA: 3'- -CG-GCUCCGCAUGA-----UGgAGUugAUgac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 122789 | 0.66 | 0.990804 |
Target: 5'- cGCCGGGGCGcUGCggGCCcuuggUCGACcGCc- -3' miRNA: 3'- -CGGCUCCGC-AUGa-UGG-----AGUUGaUGac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 3763 | 0.66 | 0.990804 |
Target: 5'- cGCCGGGGCGcUGCggGCCcuuggUCGACcGCc- -3' miRNA: 3'- -CGGCUCCGC-AUGa-UGG-----AGUUGaUGac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 29792 | 0.66 | 0.990804 |
Target: 5'- cGCCacguAGGCGUugauauGCUuccGCCUCGGC-GCUGg -3' miRNA: 3'- -CGGc---UCCGCA------UGA---UGGAGUUGaUGAC- -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 93042 | 0.66 | 0.989511 |
Target: 5'- cGCCGAauGCGUGCUGCUgc-GCUGCc- -3' miRNA: 3'- -CGGCUc-CGCAUGAUGGaguUGAUGac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 109624 | 0.66 | 0.988079 |
Target: 5'- cGCgGAGauacGCGUccACUACCUCGGCcACg- -3' miRNA: 3'- -CGgCUC----CGCA--UGAUGGAGUUGaUGac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 52430 | 0.66 | 0.988079 |
Target: 5'- gGCCGAGaaGUACUGgCUCGAgcCUGCc- -3' miRNA: 3'- -CGGCUCcgCAUGAUgGAGUU--GAUGac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 93197 | 0.66 | 0.988079 |
Target: 5'- aGCUGAGGUacgGUGCUGCC-CAGCc---- -3' miRNA: 3'- -CGGCUCCG---CAUGAUGGaGUUGaugac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 69643 | 0.66 | 0.986502 |
Target: 5'- gGCUG-GGCaGcACcgUACCUCAGCUGCUc -3' miRNA: 3'- -CGGCuCCG-CaUG--AUGGAGUUGAUGAc -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 17286 | 0.66 | 0.984768 |
Target: 5'- -aCGGGGCGgaggGCUGCC-CAuCUaACUGa -3' miRNA: 3'- cgGCUCCGCa---UGAUGGaGUuGA-UGAC- -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 120235 | 0.67 | 0.98287 |
Target: 5'- cGCCGGGGCcgguGUACcgaCUCAGCgaauugcgaUACUGg -3' miRNA: 3'- -CGGCUCCG----CAUGaugGAGUUG---------AUGAC- -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 138167 | 0.67 | 0.980798 |
Target: 5'- uCCGAGGUGgcgGCUuccggccuGCCUC-GCUGCa- -3' miRNA: 3'- cGGCUCCGCa--UGA--------UGGAGuUGAUGac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 69816 | 0.67 | 0.973462 |
Target: 5'- aGuuGAGGCcacuagccACUGCCUUGACUGCc- -3' miRNA: 3'- -CggCUCCGca------UGAUGGAGUUGAUGac -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 116410 | 0.68 | 0.97178 |
Target: 5'- gGCCGAcgucgagcgcagggcGGCGUGCaGCCUCGcgGCggacCUGg -3' miRNA: 3'- -CGGCU---------------CCGCAUGaUGGAGU--UGau--GAC- -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 55828 | 0.68 | 0.970617 |
Target: 5'- aGCCGGuGGCGgcgcgGCUgacuggGCCUCAggGCUuCUGg -3' miRNA: 3'- -CGGCU-CCGCa----UGA------UGGAGU--UGAuGAC- -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 100754 | 0.68 | 0.957049 |
Target: 5'- cGCCGAGGCGUcACgACagaaaUCGAUcggACUGa -3' miRNA: 3'- -CGGCUCCGCA-UGaUGg----AGUUGa--UGAC- -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 9676 | 0.7 | 0.929593 |
Target: 5'- gGCCuuggggGAGGCGgGCUcgcGCCUCGAC-GCUGu -3' miRNA: 3'- -CGG------CUCCGCaUGA---UGGAGUUGaUGAC- -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 128703 | 0.7 | 0.929593 |
Target: 5'- gGCCuuggggGAGGCGgGCUcgcGCCUCGAC-GCUGu -3' miRNA: 3'- -CGG------CUCCGCaUGA---UGGAGUUGaUGAC- -5' |
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24339 | 5' | -51.8 | NC_005264.1 | + | 156151 | 0.72 | 0.840016 |
Target: 5'- gGgCGAGGCGgcCgucucgGCCUCG-CUGCUGg -3' miRNA: 3'- -CgGCUCCGCauGa-----UGGAGUuGAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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