Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24340 | 3' | -53.9 | NC_005264.1 | + | 25268 | 0.66 | 0.97401 |
Target: 5'- gGCuuuuUGUCgACCGAGAUguGGgUGuGGu -3' miRNA: 3'- -UGu---ACAGgUGGCUCUGguCUgACuCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 56900 | 0.66 | 0.973206 |
Target: 5'- cCGUGUCCGCCGGGGugGGAagcgaagauggcguaGAGGg -3' miRNA: 3'- uGUACAGGUGGCUCUggUCUga-------------CUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 33972 | 0.66 | 0.971262 |
Target: 5'- cGCAagcUGUCCGCUGGcGACCAGAgc-GGGc -3' miRNA: 3'- -UGU---ACAGGUGGCU-CUGGUCUgacUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 160392 | 0.66 | 0.971262 |
Target: 5'- aACGUGUgCCGCaaggaaGAGugCAGGCUGc-- -3' miRNA: 3'- -UGUACA-GGUGg-----CUCugGUCUGACucc -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 83450 | 0.66 | 0.961779 |
Target: 5'- aGCAUGgcgCCAaaaaucgucCCGAuGAUCAgcacGACUGAGGu -3' miRNA: 3'- -UGUACa--GGU---------GGCU-CUGGU----CUGACUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 48314 | 0.67 | 0.954365 |
Target: 5'- gACGcGUCCAUUGGGAUUAGcacccCUGGGGa -3' miRNA: 3'- -UGUaCAGGUGGCUCUGGUCu----GACUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 21337 | 0.67 | 0.954365 |
Target: 5'- cGCccGUagaugCGCCGGGGCCGGA-UGAGGc -3' miRNA: 3'- -UGuaCAg----GUGGCUCUGGUCUgACUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 153105 | 0.67 | 0.950317 |
Target: 5'- uGCcUG-CCGCCGAG-UCAGAaaGAGGa -3' miRNA: 3'- -UGuACaGGUGGCUCuGGUCUgaCUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 49589 | 0.68 | 0.93177 |
Target: 5'- cGCAcG-CCGCCGGcGACCAGgACgacgGGGGg -3' miRNA: 3'- -UGUaCaGGUGGCU-CUGGUC-UGa---CUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 63133 | 0.69 | 0.890108 |
Target: 5'- cCAUGUaUCGCCGAGAUCcauuuGGACggcGAGGg -3' miRNA: 3'- uGUACA-GGUGGCUCUGG-----UCUGa--CUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 36738 | 0.7 | 0.829027 |
Target: 5'- cGCAuuccuUGcCCGCCuacuGGCCAGACUGAGa -3' miRNA: 3'- -UGU-----ACaGGUGGcu--CUGGUCUGACUCc -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 127141 | 0.71 | 0.784787 |
Target: 5'- ---cGUCCAUCG-GAaCGGGCUGAGGa -3' miRNA: 3'- uguaCAGGUGGCuCUgGUCUGACUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 8114 | 0.71 | 0.784787 |
Target: 5'- ---cGUCCAUCG-GAaCGGGCUGAGGa -3' miRNA: 3'- uguaCAGGUGGCuCUgGUCUGACUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 59796 | 0.72 | 0.756517 |
Target: 5'- cCGUGUCCGCCGcGGAgCCguacguAGACgUGAGGg -3' miRNA: 3'- uGUACAGGUGGC-UCU-GG------UCUG-ACUCC- -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 104833 | 0.76 | 0.51464 |
Target: 5'- gGCcUGUCCGCCGGGGCC--GCUGAGc -3' miRNA: 3'- -UGuACAGGUGGCUCUGGucUGACUCc -5' |
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24340 | 3' | -53.9 | NC_005264.1 | + | 156577 | 1.11 | 0.004008 |
Target: 5'- aACAUGUCCACCGAGACCAGACUGAGGa -3' miRNA: 3'- -UGUACAGGUGGCUCUGGUCUGACUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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