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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24340 | 5' | -58.6 | NC_005264.1 | + | 155094 | 0.71 | 0.543771 |
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Target: 5'- uGCCGacgcggAGAGCCCGUUUCCG-AGGaCg -3' miRNA: 3'- gCGGCg-----UCUUGGGCAGAGGCaUCCgG- -5' |
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| 24340 | 5' | -58.6 | NC_005264.1 | + | 156614 | 1.11 | 0.001599 |
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Target: 5'- cCGCCGCAGAACCCGUCUCCGUAGGCCc -3' miRNA: 3'- -GCGGCGUCUUGGGCAGAGGCAUCCGG- -5' |
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| 24340 | 5' | -58.6 | NC_005264.1 | + | 157031 | 0.66 | 0.834547 |
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Target: 5'- cCGCCGCcGGACUauugaacgCCGUGGGCg -3' miRNA: 3'- -GCGGCGuCUUGGgcaga---GGCAUCCGg -5' |
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| 24340 | 5' | -58.6 | NC_005264.1 | + | 157225 | 0.66 | 0.851757 |
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Target: 5'- gCGUCGCGcGGCCCGauagcggCUCCGgucGCCg -3' miRNA: 3'- -GCGGCGUcUUGGGCa------GAGGCaucCGG- -5' |
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| 24340 | 5' | -58.6 | NC_005264.1 | + | 158997 | 0.66 | 0.858541 |
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Target: 5'- cCGgCGCAGGACCCaugccaggagcagGUCUCCGcgcGCa -3' miRNA: 3'- -GCgGCGUCUUGGG-------------CAGAGGCaucCGg -5' |
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| 24340 | 5' | -58.6 | NC_005264.1 | + | 161647 | 0.67 | 0.791568 |
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Target: 5'- gGCCGCGGAcGCCCGguccgCUgCGUAccccaucgcgcgccGGCa -3' miRNA: 3'- gCGGCGUCU-UGGGCa----GAgGCAU--------------CCGg -5' |
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| 24340 | 5' | -58.6 | NC_005264.1 | + | 162311 | 0.71 | 0.553443 |
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Target: 5'- aCGCCGCGGGucugAUCgGUCggCC-UGGGCCa -3' miRNA: 3'- -GCGGCGUCU----UGGgCAGa-GGcAUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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