Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24341 | 3' | -58.2 | NC_005264.1 | + | 16376 | 0.67 | 0.797738 |
Target: 5'- uGC-UGgGgGGgaggGGAUGCC-CGUGGCg -3' miRNA: 3'- -CGaACgCgCCa---CCUGCGGaGUACCGa -5' |
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24341 | 3' | -58.2 | NC_005264.1 | + | 12912 | 0.7 | 0.602833 |
Target: 5'- cCUUGCGaUGGUGugcGugGCCaUCGUGGCc -3' miRNA: 3'- cGAACGC-GCCAC---CugCGG-AGUACCGa -5' |
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24341 | 3' | -58.2 | NC_005264.1 | + | 6310 | 0.68 | 0.713092 |
Target: 5'- aCUUGCGCGGc-GAgGCUUCgcGUGGCg -3' miRNA: 3'- cGAACGCGCCacCUgCGGAG--UACCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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