Results 61 - 65 of 65 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24341 | 5' | -57.6 | NC_005264.1 | + | 56802 | 0.66 | 0.824587 |
Target: 5'- -aAGUUAUGCUuuguccuGCGCUGCGCGGc-- -3' miRNA: 3'- caUCGGUACGGc------CGCGACGCGCUuau -5' |
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24341 | 5' | -57.6 | NC_005264.1 | + | 3752 | 0.66 | 0.824587 |
Target: 5'- -gGGCCucuUGCCgccggGGCGCUGCGgGc--- -3' miRNA: 3'- caUCGGu--ACGG-----CCGCGACGCgCuuau -5' |
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24341 | 5' | -57.6 | NC_005264.1 | + | 87153 | 0.66 | 0.815958 |
Target: 5'- --cGCCGaaagaCGGCGCUGCGCGc--- -3' miRNA: 3'- cauCGGUacg--GCCGCGACGCGCuuau -5' |
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24341 | 5' | -57.6 | NC_005264.1 | + | 131720 | 0.66 | 0.815958 |
Target: 5'- -cGGCguUGCUGGCGCUcugGCGCu---- -3' miRNA: 3'- caUCGguACGGCCGCGA---CGCGcuuau -5' |
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24341 | 5' | -57.6 | NC_005264.1 | + | 79973 | 0.66 | 0.83637 |
Target: 5'- -cGGCCAgcaaacagagaccgaUGCCGGC-C-GCGCGGAa- -3' miRNA: 3'- caUCGGU---------------ACGGCCGcGaCGCGCUUau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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