miRNA: 3'- cauCGGUACGGC-CGCGa-----------CGCGCUuau -5'
miRNA: 3'- cAUCGgUACGGCCG---CGACGCGCUuau -5'
miRNA: 3'- cauCGGuaCGGCCGCGACGCgCUUau -5'
miRNA: 3'- -CAUCGGuaCGGCCGCGa--CGCGCUuau -5'
miRNA: 3'- -CAUCGGUACGGCCGCGACGCGCUUAU- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24341 | 5' | -57.6 | NC_005264.1 | + | 150862 | 0.72 | 0.478015 |
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Target: 5'- --uGCCGUGCCGcGCGCggcaagacauggGCGCGAc-- -3' miRNA: 3'- cauCGGUACGGC-CGCGa-----------CGCGCUuau -5' |
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| 24341 | 5' | -57.6 | NC_005264.1 | + | 137936 | 0.72 | 0.470449 |
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Target: 5'- uUGGUgGUGCCGGUaucGUUGCGCGAc-- -3' miRNA: 3'- cAUCGgUACGGCCG---CGACGCGCUuau -5' |
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| 24341 | 5' | -57.6 | NC_005264.1 | + | 25606 | 0.72 | 0.461079 |
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Target: 5'- --cGCCggGCCGGCGUUGCG-GAAc- -3' miRNA: 3'- cauCGGuaCGGCCGCGACGCgCUUau -5' |
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| 24341 | 5' | -57.6 | NC_005264.1 | + | 103133 | 0.75 | 0.311745 |
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Target: 5'- gGUAGCC--GCCGGCGCggcGCGCGGc-- -3' miRNA: 3'- -CAUCGGuaCGGCCGCGa--CGCGCUuau -5' |
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| 24341 | 5' | -57.6 | NC_005264.1 | + | 156065 | 1.06 | 0.002789 |
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Target: 5'- gGUAGCCAUGCCGGCGCUGCGCGAAUAc -3' miRNA: 3'- -CAUCGGUACGGCCGCGACGCGCUUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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