Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24362 | 5' | -53.6 | NC_005264.1 | + | 124231 | 0.66 | 0.964814 |
Target: 5'- uGUCUCGCGccgcGGCGAGGCGaagcuCUUCu- -3' miRNA: 3'- -CAGAGCGCcau-UCGCUCUGC-----GAAGua -5' |
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24362 | 5' | -53.6 | NC_005264.1 | + | 21519 | 0.66 | 0.964814 |
Target: 5'- --gUCGCaGUGGGCGcagguguaguuGACGCUUCGUg -3' miRNA: 3'- cagAGCGcCAUUCGCu----------CUGCGAAGUA- -5' |
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24362 | 5' | -53.6 | NC_005264.1 | + | 5204 | 0.66 | 0.964814 |
Target: 5'- uGUCUCGCGccgcGGCGAGGCGaagcuCUUCu- -3' miRNA: 3'- -CAGAGCGCcau-UCGCUCUGC-----GAAGua -5' |
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24362 | 5' | -53.6 | NC_005264.1 | + | 29764 | 0.66 | 0.957659 |
Target: 5'- uUCUCGCGGUA---GGGAUGCUgUCGg -3' miRNA: 3'- cAGAGCGCCAUucgCUCUGCGA-AGUa -5' |
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24362 | 5' | -53.6 | NC_005264.1 | + | 25859 | 0.68 | 0.913514 |
Target: 5'- cGUCUCGCuuaccGCGAGACGCU-CGa -3' miRNA: 3'- -CAGAGCGccauuCGCUCUGCGAaGUa -5' |
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24362 | 5' | -53.6 | NC_005264.1 | + | 36809 | 0.74 | 0.626555 |
Target: 5'- uGUCUCuGCGGcgcAGGCGAGGCGCggCGa -3' miRNA: 3'- -CAGAG-CGCCa--UUCGCUCUGCGaaGUa -5' |
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24362 | 5' | -53.6 | NC_005264.1 | + | 136985 | 1.07 | 0.006571 |
Target: 5'- cGUCUCGCGGUAAGCGAGACGCUUCAUg -3' miRNA: 3'- -CAGAGCGCCAUUCGCUCUGCGAAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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