miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
24362 5' -53.6 NC_005264.1 + 124231 0.66 0.964814
Target:  5'- uGUCUCGCGccgcGGCGAGGCGaagcuCUUCu- -3'
miRNA:   3'- -CAGAGCGCcau-UCGCUCUGC-----GAAGua -5'
24362 5' -53.6 NC_005264.1 + 21519 0.66 0.964814
Target:  5'- --gUCGCaGUGGGCGcagguguaguuGACGCUUCGUg -3'
miRNA:   3'- cagAGCGcCAUUCGCu----------CUGCGAAGUA- -5'
24362 5' -53.6 NC_005264.1 + 5204 0.66 0.964814
Target:  5'- uGUCUCGCGccgcGGCGAGGCGaagcuCUUCu- -3'
miRNA:   3'- -CAGAGCGCcau-UCGCUCUGC-----GAAGua -5'
24362 5' -53.6 NC_005264.1 + 29764 0.66 0.957659
Target:  5'- uUCUCGCGGUA---GGGAUGCUgUCGg -3'
miRNA:   3'- cAGAGCGCCAUucgCUCUGCGA-AGUa -5'
24362 5' -53.6 NC_005264.1 + 25859 0.68 0.913514
Target:  5'- cGUCUCGCuuaccGCGAGACGCU-CGa -3'
miRNA:   3'- -CAGAGCGccauuCGCUCUGCGAaGUa -5'
24362 5' -53.6 NC_005264.1 + 36809 0.74 0.626555
Target:  5'- uGUCUCuGCGGcgcAGGCGAGGCGCggCGa -3'
miRNA:   3'- -CAGAG-CGCCa--UUCGCUCUGCGaaGUa -5'
24362 5' -53.6 NC_005264.1 + 136985 1.07 0.006571
Target:  5'- cGUCUCGCGGUAAGCGAGACGCUUCAUg -3'
miRNA:   3'- -CAGAGCGCCAUUCGCUCUGCGAAGUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.