Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24369 | 5' | -49.8 | NC_005264.1 | + | 20840 | 0.69 | 0.973941 |
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Target: 5'- -gCGAGAugcCGUAUuccACAGUCUGGaGCc -3' miRNA: 3'- uaGCUCUu--GCGUAua-UGUCAGACC-CG- -5' |
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| 24369 | 5' | -49.8 | NC_005264.1 | + | 25724 | 0.68 | 0.987014 |
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Target: 5'- uUCGAGAcCGCGUAc-CAGUUaGGGUc -3' miRNA: 3'- uAGCUCUuGCGUAUauGUCAGaCCCG- -5' |
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| 24369 | 5' | -49.8 | NC_005264.1 | + | 156160 | 0.68 | 0.987014 |
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Target: 5'- -aCGaAGGGCGCAUAUgcGCGGcUUUGcGGCg -3' miRNA: 3'- uaGC-UCUUGCGUAUA--UGUC-AGAC-CCG- -5' |
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| 24369 | 5' | -49.8 | NC_005264.1 | + | 45171 | 0.68 | 0.988579 |
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Target: 5'- --gGAGAGCGCGgcagcgaACGGUCUGG-Ca -3' miRNA: 3'- uagCUCUUGCGUaua----UGUCAGACCcG- -5' |
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| 24369 | 5' | -49.8 | NC_005264.1 | + | 43980 | 0.68 | 0.989993 |
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Target: 5'- -aUGAGGACGCAgaacgcgagGCGGaCUuGGGCa -3' miRNA: 3'- uaGCUCUUGCGUaua------UGUCaGA-CCCG- -5' |
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| 24369 | 5' | -49.8 | NC_005264.1 | + | 93166 | 0.67 | 0.994335 |
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Target: 5'- cGUCG-GGGCGCcgcucCAGaCUGGGCg -3' miRNA: 3'- -UAGCuCUUGCGuauauGUCaGACCCG- -5' |
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| 24369 | 5' | -49.8 | NC_005264.1 | + | 125528 | 0.67 | 0.994335 |
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Target: 5'- uGUCGAGAgaagGCGgGUGUugcuGCGG-CUGcGGCg -3' miRNA: 3'- -UAGCUCU----UGCgUAUA----UGUCaGAC-CCG- -5' |
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| 24369 | 5' | -49.8 | NC_005264.1 | + | 22320 | 0.67 | 0.995138 |
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Target: 5'- aGUCuGGcGCGCGgg-GCaaugGGUCUGGGCu -3' miRNA: 3'- -UAGcUCuUGCGUauaUG----UCAGACCCG- -5' |
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| 24369 | 5' | -49.8 | NC_005264.1 | + | 129943 | 1.12 | 0.007563 |
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Target: 5'- gAUCGAGAACGCAUAUACAGUCUGGGCg -3' miRNA: 3'- -UAGCUCUUGCGUAUAUGUCAGACCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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