Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24377 | 5' | -58.4 | NC_005264.1 | + | 115290 | 0.67 | 0.782198 |
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Target: 5'- cGCGAcaacgaCGUCCCG-CGCagccCUUCCUGAc -3' miRNA: 3'- aCGCUa-----GCAGGGCaGCGc---GAAGGGCU- -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 125167 | 0.69 | 0.697491 |
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Target: 5'- gGCGAgggccgCGcgCCCGUCGCGCaUCgCGu -3' miRNA: 3'- aCGCUa-----GCa-GGGCAGCGCGaAGgGCu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 126672 | 1.09 | 0.002227 |
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Target: 5'- uUGCGAUCGUCCCGUCGCGCUUCCCGAg -3' miRNA: 3'- -ACGCUAGCAGGGCAGCGCGAAGGGCU- -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 134766 | 0.66 | 0.849117 |
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Target: 5'- cGCGAguaagcccguguUCGcUUCCG-CGCGCUUuuaCCCGGc -3' miRNA: 3'- aCGCU------------AGC-AGGGCaGCGCGAA---GGGCU- -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 134868 | 0.72 | 0.501986 |
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Target: 5'- gGCGGUCGUCgUcaCGCGUUUCCCa- -3' miRNA: 3'- aCGCUAGCAGgGcaGCGCGAAGGGcu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 143752 | 0.67 | 0.794606 |
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Target: 5'- cGCGGUgcaGUCCUggcucccuaugcgcgGgaagCGCGCUUCCCGc -3' miRNA: 3'- aCGCUAg--CAGGG---------------Ca---GCGCGAAGGGCu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 147372 | 0.68 | 0.745456 |
|
Target: 5'- cGCGAUCG-CCgcuuucgcguCGUCGCGCUUgUCCa- -3' miRNA: 3'- aCGCUAGCaGG----------GCAGCGCGAA-GGGcu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 149248 | 0.68 | 0.754802 |
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Target: 5'- cGCGAUCGccggUCCCGUCuucguuccGCcCUUCCCc- -3' miRNA: 3'- aCGCUAGC----AGGGCAG--------CGcGAAGGGcu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 161699 | 0.68 | 0.736016 |
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Target: 5'- gGCcgGAUCGUCCCGU-GCGCccgUCCGu -3' miRNA: 3'- aCG--CUAGCAGGGCAgCGCGaa-GGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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