Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24377 | 5' | -58.4 | NC_005264.1 | + | 6930 | 0.68 | 0.736016 |
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Target: 5'- cGCGAUUGUCUugagcuaauaCGUCGCGCcgCaCCGu -3' miRNA: 3'- aCGCUAGCAGG----------GCAGCGCGaaG-GGCu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 53722 | 0.68 | 0.736016 |
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Target: 5'- gGCGAacgagucCGUCUCGUCGUGCccgUCuCCGGc -3' miRNA: 3'- aCGCUa------GCAGGGCAGCGCGa--AG-GGCU- -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 149248 | 0.68 | 0.754802 |
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Target: 5'- cGCGAUCGccggUCCCGUCuucguuccGCcCUUCCCc- -3' miRNA: 3'- aCGCUAGC----AGGGCAG--------CGcGAAGGGcu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 63849 | 0.67 | 0.788435 |
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Target: 5'- cGCGGUCGUUUCGUCaggcCGCUcacaacggccgucuUCUCGAg -3' miRNA: 3'- aCGCUAGCAGGGCAGc---GCGA--------------AGGGCU- -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 34451 | 0.67 | 0.791088 |
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Target: 5'- gGCGAucaagcUCGUCCCGcacuauuugcgCGCGCUUCagGAg -3' miRNA: 3'- aCGCU------AGCAGGGCa----------GCGCGAAGggCU- -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 100584 | 0.67 | 0.816078 |
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Target: 5'- aGCGccGUCGUuguacagCCUGUgGCgGCUUCCCGu -3' miRNA: 3'- aCGC--UAGCA-------GGGCAgCG-CGAAGGGCu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 53205 | 0.66 | 0.825222 |
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Target: 5'- cGCGAgUUGcaaCCGUCGCGCccCCCGc -3' miRNA: 3'- aCGCU-AGCag-GGCAGCGCGaaGGGCu -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 3242 | 0.66 | 0.848347 |
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Target: 5'- gUGCGcauguccGUCG-CUCGUCuCGCUUCUCGGu -3' miRNA: 3'- -ACGC-------UAGCaGGGCAGcGCGAAGGGCU- -5' |
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| 24377 | 5' | -58.4 | NC_005264.1 | + | 62016 | 0.71 | 0.59867 |
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Target: 5'- cGCcGUCGUCCCuGUCGCGCUcuagcaaggUUCgGAg -3' miRNA: 3'- aCGcUAGCAGGG-CAGCGCGA---------AGGgCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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