Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 24381 | 5' | -50 | NC_005264.1 | + | 43896 | 0.66 | 0.995941 |
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Target: 5'- ----gGGGCGccGGAGAcGAuAGCGCGg -3' miRNA: 3'- uaguaUCUGCauCCUCU-CUuUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 15430 | 0.66 | 0.996551 |
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Target: 5'- cUCGUGGcGCGaUGGGAGcucaugaguguGGAcAAGCGCGa -3' miRNA: 3'- uAGUAUC-UGC-AUCCUC-----------UCU-UUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 38165 | 0.66 | 0.997275 |
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Target: 5'- uUCAgaUAGugG-AGGGGGGAAAuaucguaaaguccuuGCGCGu -3' miRNA: 3'- uAGU--AUCugCaUCCUCUCUUU---------------CGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 161704 | 0.66 | 0.997501 |
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Target: 5'- gAUCGUcc-CGUgcgcccguccgucAGaGAGAGAGAGCGCGa -3' miRNA: 3'- -UAGUAucuGCA-------------UC-CUCUCUUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 48668 | 0.66 | 0.997544 |
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Target: 5'- -aCGggGGGCG-AGGAGAucuGGGCGCGa -3' miRNA: 3'- uaGUa-UCUGCaUCCUCUcu-UUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 42678 | 0.66 | 0.997501 |
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Target: 5'- gAUCGUcc-CGUgcgcccguccgucAGaGAGAGAGAGCGCGa -3' miRNA: 3'- -UAGUAucuGCA-------------UC-CUCUCUUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 54113 | 0.66 | 0.996551 |
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Target: 5'- ----aAGAUGUcaguGGAGAGuucGAGCGCGc -3' miRNA: 3'- uaguaUCUGCAu---CCUCUCu--UUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 155277 | 0.66 | 0.995941 |
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Target: 5'- --gGUAGGCGgcGGGGAcGAugauguAGCGUGg -3' miRNA: 3'- uagUAUCUGCauCCUCU-CUu-----UCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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