Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24381 | 5' | -50 | NC_005264.1 | + | 124354 | 1.09 | 0.011449 |
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Target: 5'- cAUCAUAGACGUAGGAGAGAAAGCGCGg -3' miRNA: 3'- -UAGUAUCUGCAUCCUCUCUUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 132325 | 0.67 | 0.991444 |
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Target: 5'- gGUCGUggagcuccccggaGGACGgccgcgcggagcaggAGGAGAG-GGGCGCGa -3' miRNA: 3'- -UAGUA-------------UCUGCa--------------UCCUCUCuUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 154209 | 0.67 | 0.992566 |
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Target: 5'- -aCGUAGACGUGGcGGuG-AAGCGCc -3' miRNA: 3'- uaGUAUCUGCAUCcUCuCuUUCGCGc -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 154793 | 0.68 | 0.987098 |
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Target: 5'- -----cGACGUGGGuGAagaggcgGAGGGCGCGg -3' miRNA: 3'- uaguauCUGCAUCCuCU-------CUUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 155277 | 0.66 | 0.995941 |
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Target: 5'- --gGUAGGCGgcGGGGAcGAugauguAGCGUGg -3' miRNA: 3'- uagUAUCUGCauCCUCU-CUu-----UCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 155314 | 0.67 | 0.994459 |
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Target: 5'- -gCGUAGGCG-AGGAGAG--GGCGg- -3' miRNA: 3'- uaGUAUCUGCaUCCUCUCuuUCGCgc -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 161704 | 0.66 | 0.997501 |
|
Target: 5'- gAUCGUcc-CGUgcgcccguccgucAGaGAGAGAGAGCGCGa -3' miRNA: 3'- -UAGUAucuGCA-------------UC-CUCUCUUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 162179 | 0.67 | 0.993568 |
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Target: 5'- --gGUGGAgCG-GGGAgGGGggGGCGCGu -3' miRNA: 3'- uagUAUCU-GCaUCCU-CUCuuUCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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