Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24381 | 5' | -50 | NC_005264.1 | + | 42678 | 0.66 | 0.997501 |
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Target: 5'- gAUCGUcc-CGUgcgcccguccgucAGaGAGAGAGAGCGCGa -3' miRNA: 3'- -UAGUAucuGCA-------------UC-CUCUCUUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 38165 | 0.66 | 0.997275 |
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Target: 5'- uUCAgaUAGugG-AGGGGGGAAAuaucguaaaguccuuGCGCGu -3' miRNA: 3'- uAGU--AUCugCaUCCUCUCUUU---------------CGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 36287 | 0.67 | 0.994459 |
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Target: 5'- -gCGUAGGCG-AGGAGAG--GGCGg- -3' miRNA: 3'- uaGUAUCUGCaUCCUCUCuuUCGCgc -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 35767 | 0.68 | 0.987098 |
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Target: 5'- -----cGACGUGGGuGAagaggcgGAGGGCGCGg -3' miRNA: 3'- uaguauCUGCAUCCuCU-------CUUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 20517 | 0.68 | 0.985383 |
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Target: 5'- uUCGUAGACGUcucuguguccggcGGGcacGAaGAAGCGCGa -3' miRNA: 3'- uAGUAUCUGCA-------------UCCu--CUcUUUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 19031 | 0.69 | 0.979423 |
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Target: 5'- cGUgAUGGACGgcAGGcGGGAAAGCGUu -3' miRNA: 3'- -UAgUAUCUGCa-UCCuCUCUUUCGCGc -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 15430 | 0.66 | 0.996551 |
|
Target: 5'- cUCGUGGcGCGaUGGGAGcucaugaguguGGAcAAGCGCGa -3' miRNA: 3'- uAGUAUC-UGC-AUCCUC-----------UCU-UUCGCGC- -5' |
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| 24381 | 5' | -50 | NC_005264.1 | + | 13298 | 0.67 | 0.991444 |
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Target: 5'- gGUCGUggagcuccccggaGGACGgccgcgcggagcaggAGGAGAG-GGGCGCGa -3' miRNA: 3'- -UAGUA-------------UCUGCa--------------UCCUCUCuUUCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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