Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 24382 | 5' | -57.6 | NC_005264.1 | + | 137859 | 0.67 | 0.811689 |
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Target: 5'- -gGGGGUacggggacaACCGCAgGUCuCGCGGgAGc -3' miRNA: 3'- aaCCCCA---------UGGCGUgCAG-GUGCUgUCa -5' |
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| 24382 | 5' | -57.6 | NC_005264.1 | + | 91173 | 0.67 | 0.82028 |
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Target: 5'- -cGGGGUGCagcaaacacgUGUACG-CCACGcACAGg -3' miRNA: 3'- aaCCCCAUG----------GCGUGCaGGUGC-UGUCa -5' |
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| 24382 | 5' | -57.6 | NC_005264.1 | + | 45411 | 0.66 | 0.845015 |
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Target: 5'- -gGGcGGcGCCcuGCGCG-CCGCGACAGc -3' miRNA: 3'- aaCC-CCaUGG--CGUGCaGGUGCUGUCa -5' |
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| 24382 | 5' | -57.6 | NC_005264.1 | + | 111525 | 0.66 | 0.852889 |
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Target: 5'- -cGGGGUacaaguggcacGCCG-ACGUgCACGAgGGUg -3' miRNA: 3'- aaCCCCA-----------UGGCgUGCAgGUGCUgUCA- -5' |
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| 24382 | 5' | -57.6 | NC_005264.1 | + | 152387 | 0.66 | 0.852889 |
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Target: 5'- -cGGcGGUcacuCUGCccuCGUCUACGACAGg -3' miRNA: 3'- aaCC-CCAu---GGCGu--GCAGGUGCUGUCa -5' |
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| 24382 | 5' | -57.6 | NC_005264.1 | + | 100501 | 0.66 | 0.859808 |
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Target: 5'- -aGGGGUugCGgGCGaaagCCcucauagACGACAGg -3' miRNA: 3'- aaCCCCAugGCgUGCa---GG-------UGCUGUCa -5' |
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| 24382 | 5' | -57.6 | NC_005264.1 | + | 32926 | 0.66 | 0.83695 |
|
Target: 5'- -aGGGGUcGCgGgGCG-CCGCGugAGUc -3' miRNA: 3'- aaCCCCA-UGgCgUGCaGGUGCugUCA- -5' |
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| 24382 | 5' | -57.6 | NC_005264.1 | + | 118038 | 0.66 | 0.860566 |
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Target: 5'- aUGGaGGUuuGCCGCgGCGaCCGCGGCGu- -3' miRNA: 3'- aACC-CCA--UGGCG-UGCaGGUGCUGUca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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