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miRNA: 3'- cUCGCCa---AGGGGG---UCGGGGGUUUgug -5'
miRNA: 3'- cuCGCCaAGGGGgUCGGGGGUUUGUG- -5'
miRNA: 3'- cUCGCCaaGGGGGUCGGg--GGUUUGUG- -5'
miRNA: 3'- -CUCGCCAAGGGGGUCGGGGGUUUGUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 24384 | 3' | -59.5 | NC_005264.1 | + | 97372 | 0.73 | 0.380338 |
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Target: 5'- cAGCGGcguaUCCCCCaaaAGCCCCCAGGg-- -3' miRNA: 3'- cUCGCCa---AGGGGG---UCGGGGGUUUgug -5' |
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| 24384 | 3' | -59.5 | NC_005264.1 | + | 27306 | 0.76 | 0.230725 |
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Target: 5'- -cGCGG-UCCCUaGGUCCCCGAGCACu -3' miRNA: 3'- cuCGCCaAGGGGgUCGGGGGUUUGUG- -5' |
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| 24384 | 3' | -59.5 | NC_005264.1 | + | 104930 | 0.78 | 0.167951 |
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Target: 5'- cGGCGGaaCCCCCAGCCgugCCAAGCGCc -3' miRNA: 3'- cUCGCCaaGGGGGUCGGg--GGUUUGUG- -5' |
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| 24384 | 3' | -59.5 | NC_005264.1 | + | 121273 | 1.1 | 0.00117 |
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Target: 5'- cGAGCGGUUCCCCCAGCCCCCAAACACg -3' miRNA: 3'- -CUCGCCAAGGGGGUCGGGGGUUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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