Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24384 | 3' | -59.5 | NC_005264.1 | + | 127661 | 0.67 | 0.692548 |
Target: 5'- cGGGCuGGggacgCCCCgAaccuuGCCCaCCAGACACa -3' miRNA: 3'- -CUCG-CCaa---GGGGgU-----CGGG-GGUUUGUG- -5' |
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24384 | 3' | -59.5 | NC_005264.1 | + | 1950 | 0.67 | 0.681618 |
Target: 5'- -cGCGGccguugCCCCCccccccgAGCUCCCAuauGCACu -3' miRNA: 3'- cuCGCCaa----GGGGG-------UCGGGGGUu--UGUG- -5' |
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24384 | 3' | -59.5 | NC_005264.1 | + | 30340 | 0.67 | 0.671641 |
Target: 5'- cGAGCuG-UCCCCCgAGUCCCCGcaggagccccuucGGCGCc -3' miRNA: 3'- -CUCGcCaAGGGGG-UCGGGGGU-------------UUGUG- -5' |
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24384 | 3' | -59.5 | NC_005264.1 | + | 19248 | 0.66 | 0.778729 |
Target: 5'- -cGCGccauucucGUUCCCuCCGGgCCCCGGGgACg -3' miRNA: 3'- cuCGC--------CAAGGG-GGUCgGGGGUUUgUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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