Results 81 - 87 of 87 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24385 | 3' | -57.9 | NC_005264.1 | + | 2926 | 0.67 | 0.806037 |
|
Target: 5'- gGAGUcGCCG-AGCGagaCGGACGgGAAGa -3' miRNA: 3'- gCUCA-CGGCgUCGCg--GCUUGCgCUUU- -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 87755 | 0.67 | 0.797199 |
|
Target: 5'- gCGccUGCCGCAGCGCC----GCGAAGa -3' miRNA: 3'- -GCucACGGCGUCGCGGcuugCGCUUU- -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 67252 | 0.67 | 0.797199 |
|
Target: 5'- gGGGUaGCCGCuaaagcGCGCUGugguauagacGACGCGAAu -3' miRNA: 3'- gCUCA-CGGCGu-----CGCGGC----------UUGCGCUUu -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 10525 | 0.67 | 0.788215 |
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Target: 5'- gCGGGU-CCuguGCGGCGCCGcccucaggGGCGCGGAu -3' miRNA: 3'- -GCUCAcGG---CGUCGCGGC--------UUGCGCUUu -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 57367 | 0.67 | 0.782758 |
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Target: 5'- uGGGUGCCGUguuaaccuucgguggGGCgcaGCCGAuuCGCGAu- -3' miRNA: 3'- gCUCACGGCG---------------UCG---CGGCUu-GCGCUuu -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 153881 | 0.67 | 0.779093 |
|
Target: 5'- -uGGUGCUGCAGCGaguuggccggcCCGAAgGcCGAGAa -3' miRNA: 3'- gcUCACGGCGUCGC-----------GGCUUgC-GCUUU- -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 129312 | 0.66 | 0.847754 |
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Target: 5'- ----cGCgGCAGCGCCGAcccuAUGgGAAAg -3' miRNA: 3'- gcucaCGgCGUCGCGGCU----UGCgCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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