Results 81 - 87 of 87 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24385 | 3' | -57.9 | NC_005264.1 | + | 158246 | 0.68 | 0.731723 |
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Target: 5'- ----cGCCGCGGCGCUG-GCGgGGAGg -3' miRNA: 3'- gcucaCGGCGUCGCGGCuUGCgCUUU- -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 118040 | 0.68 | 0.741402 |
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Target: 5'- gGAGguuUGCCGCGGCGaCCGcGGCGuCGGc- -3' miRNA: 3'- gCUC---ACGGCGUCGC-GGC-UUGC-GCUuu -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 159988 | 0.68 | 0.750987 |
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Target: 5'- ----aGCCGCAGCgggcgacgGCgCGAGCGCGAc- -3' miRNA: 3'- gcucaCGGCGUCG--------CG-GCUUGCGCUuu -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 122300 | 0.67 | 0.769842 |
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Target: 5'- ----aGCCGCAGCGCCacgucccuGGGCGaCGggGa -3' miRNA: 3'- gcucaCGGCGUCGCGG--------CUUGC-GCuuU- -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 113044 | 0.67 | 0.779093 |
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Target: 5'- gCGAcuggGCCGCGGCGaUCuuACGCGAGAc -3' miRNA: 3'- -GCUca--CGGCGUCGC-GGcuUGCGCUUU- -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 16432 | 0.67 | 0.787309 |
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Target: 5'- uCGAcUGCCGCGGgGCCugcccgcggguguGAACGUGGu- -3' miRNA: 3'- -GCUcACGGCGUCgCGG-------------CUUGCGCUuu -5' |
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| 24385 | 3' | -57.9 | NC_005264.1 | + | 150865 | 0.66 | 0.831593 |
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Target: 5'- --cGUGCCGC-GCGCggcaagacaUGGGCGCGAc- -3' miRNA: 3'- gcuCACGGCGuCGCG---------GCUUGCGCUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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