Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24391 | 3' | -54.3 | NC_005264.1 | + | 79214 | 0.69 | 0.814587 |
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Target: 5'- -gGGGCuagGCUGCGUGCAGGcgGCGg -3' miRNA: 3'- agCCCGcuaUGAUGCGCGUUCa-CGUa -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 161454 | 0.69 | 0.814587 |
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Target: 5'- aCGGGa-AUGCgacGCGCGCAGGaUGCAUc -3' miRNA: 3'- aGCCCgcUAUGa--UGCGCGUUC-ACGUA- -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 28353 | 0.71 | 0.758679 |
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Target: 5'- gCGGGCGAc---ACGCGCAauuggAGUGCGg -3' miRNA: 3'- aGCCCGCUaugaUGCGCGU-----UCACGUa -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 29478 | 0.71 | 0.729028 |
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Target: 5'- -gGGGCGGUGCa--GCGCAGGgcuagGCAa -3' miRNA: 3'- agCCCGCUAUGaugCGCGUUCa----CGUa -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 52103 | 0.72 | 0.678001 |
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Target: 5'- -aGGGCGAcgucagACauaGCGCGUGAGUGCGUg -3' miRNA: 3'- agCCCGCUa-----UGa--UGCGCGUUCACGUA- -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 76350 | 0.73 | 0.636415 |
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Target: 5'- gCGcGGCGA--CUGCGCGCAGGcGCGUu -3' miRNA: 3'- aGC-CCGCUauGAUGCGCGUUCaCGUA- -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 48617 | 0.73 | 0.605157 |
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Target: 5'- uUCGGGUGGUACcGCuucaaaccuuGCGCGGGUGCc- -3' miRNA: 3'- -AGCCCGCUAUGaUG----------CGCGUUCACGua -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 130551 | 0.75 | 0.523238 |
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Target: 5'- gUCGcGGCGcguGUGCUACGCGCAGGUcucGCu- -3' miRNA: 3'- -AGC-CCGC---UAUGAUGCGCGUUCA---CGua -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 118541 | 1.07 | 0.00518 |
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Target: 5'- aUCGGGCGAUACUACGCGCAAGUGCAUg -3' miRNA: 3'- -AGCCCGCUAUGAUGCGCGUUCACGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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