Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24391 | 3' | -54.3 | NC_005264.1 | + | 52853 | 0.67 | 0.912486 |
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Target: 5'- gCGGGa---GCUGCGCGCAuacgacGUGCAc -3' miRNA: 3'- aGCCCgcuaUGAUGCGCGUu-----CACGUa -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 52968 | 0.68 | 0.893262 |
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Target: 5'- aCGcGGCGGUGCUGCGaacucagGCG-GUGCGc -3' miRNA: 3'- aGC-CCGCUAUGAUGCg------CGUuCACGUa -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 98099 | 0.67 | 0.912486 |
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Target: 5'- -aGGGCGAUcCU-UGCGCGGG-GCAc -3' miRNA: 3'- agCCCGCUAuGAuGCGCGUUCaCGUa -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 7602 | 0.67 | 0.924077 |
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Target: 5'- gCGGGCucuGGU-CUGCGCGCA--UGCGg -3' miRNA: 3'- aGCCCG---CUAuGAUGCGCGUucACGUa -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 123507 | 0.67 | 0.9295 |
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Target: 5'- cUGGGCGAUguGCU-CGCGgugucCAAGUGCc- -3' miRNA: 3'- aGCCCGCUA--UGAuGCGC-----GUUCACGua -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 48672 | 0.66 | 0.939604 |
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Target: 5'- -gGGGCGAggagauCUGgGCGCGAGUcuGCu- -3' miRNA: 3'- agCCCGCUau----GAUgCGCGUUCA--CGua -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 44149 | 0.66 | 0.948725 |
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Target: 5'- -aGGGCaGGUAagcCGCGCGAGcGCGUa -3' miRNA: 3'- agCCCG-CUAUgauGCGCGUUCaCGUA- -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 35499 | 0.66 | 0.948725 |
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Target: 5'- aCGacGGCGAcGCcGCgGCGCAGGUGCc- -3' miRNA: 3'- aGC--CCGCUaUGaUG-CGCGUUCACGua -5' |
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| 24391 | 3' | -54.3 | NC_005264.1 | + | 118541 | 1.07 | 0.00518 |
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Target: 5'- aUCGGGCGAUACUACGCGCAAGUGCAUg -3' miRNA: 3'- -AGCCCGCUAUGAUGCGCGUUCACGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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