Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24395 | 5' | -62.9 | NC_005264.1 | + | 126558 | 0.66 | 0.60993 |
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Target: 5'- cGCCcucUCCUCGCCUaCGCAcGaGGACGGc -3' miRNA: 3'- uCGGc--AGGAGCGGG-GCGU-C-CCUGUCu -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 131544 | 0.68 | 0.477855 |
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Target: 5'- aGGUCGUCCUCGCugcaucuagCCgGUAGGaGCGGGg -3' miRNA: 3'- -UCGGCAGGAGCG---------GGgCGUCCcUGUCU- -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 136683 | 0.66 | 0.590521 |
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Target: 5'- uGCCGcgaaagUCCUCGCCgCgGCcuGGGACAu- -3' miRNA: 3'- uCGGC------AGGAGCGG-GgCGu-CCCUGUcu -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 143579 | 0.66 | 0.6294 |
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Target: 5'- uGCCGUCCcCGgggCCCGgAGGGAacgAGAa -3' miRNA: 3'- uCGGCAGGaGCg--GGGCgUCCCUg--UCU- -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 146346 | 0.71 | 0.324906 |
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Target: 5'- cGCCGgcgugCCUCGaCCCCGCAGGccaccGCGGc -3' miRNA: 3'- uCGGCa----GGAGC-GGGGCGUCCc----UGUCu -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 152111 | 0.66 | 0.600215 |
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Target: 5'- uGCCGUCggUGUCCgCGCcgGGGGACAa- -3' miRNA: 3'- uCGGCAGgaGCGGG-GCG--UCCCUGUcu -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 155301 | 0.68 | 0.514422 |
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Target: 5'- aGGCCGUCCUCG-UgCGUAGGcgaggagagGGCGGAc -3' miRNA: 3'- -UCGGCAGGAGCgGgGCGUCC---------CUGUCU- -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 160450 | 0.68 | 0.514422 |
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Target: 5'- aGGCCGgcgCCggGCuCCUGCGGGcGACGGc -3' miRNA: 3'- -UCGGCa--GGagCG-GGGCGUCC-CUGUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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