Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24395 | 5' | -62.9 | NC_005264.1 | + | 126558 | 0.66 | 0.60993 |
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Target: 5'- cGCCcucUCCUCGCCUaCGCAcGaGGACGGc -3' miRNA: 3'- uCGGc--AGGAGCGGG-GCGU-C-CCUGUCu -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 136683 | 0.66 | 0.590521 |
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Target: 5'- uGCCGcgaaagUCCUCGCCgCgGCcuGGGACAu- -3' miRNA: 3'- uCGGC------AGGAGCGG-GgCGu-CCCUGUcu -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 125289 | 0.66 | 0.590521 |
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Target: 5'- cGCgGUCgUCGCCgCCGUcGGGAggcaaaCAGAu -3' miRNA: 3'- uCGgCAGgAGCGG-GGCGuCCCU------GUCU- -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 125590 | 0.66 | 0.600215 |
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Target: 5'- cGCCucGUCCacggugaugCGCCCCcCAGGGGCcGAc -3' miRNA: 3'- uCGG--CAGGa--------GCGGGGcGUCCCUGuCU- -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 994 | 0.67 | 0.541629 |
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Target: 5'- aAGCU---CUUGCCCCGCgcccgugugguggGGGGGCAGGg -3' miRNA: 3'- -UCGGcagGAGCGGGGCG-------------UCCCUGUCU- -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 21087 | 0.68 | 0.523744 |
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Target: 5'- uGCCGUgCUCGCCuCCG-AGGGcCuGAc -3' miRNA: 3'- uCGGCAgGAGCGG-GGCgUCCCuGuCU- -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 160450 | 0.68 | 0.514422 |
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Target: 5'- aGGCCGgcgCCggGCuCCUGCGGGcGACGGc -3' miRNA: 3'- -UCGGCa--GGagCG-GGGCGUCC-CUGUCu -5' |
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| 24395 | 5' | -62.9 | NC_005264.1 | + | 146346 | 0.71 | 0.324906 |
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Target: 5'- cGCCGgcgugCCUCGaCCCCGCAGGccaccGCGGc -3' miRNA: 3'- uCGGCa----GGAGC-GGGGCGUCCc----UGUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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