Results 101 - 106 of 106 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 24398 | 5' | -59.2 | NC_005264.1 | + | 89072 | 0.66 | 0.76417 |
|
Target: 5'- cUCGguGGCGGACAugauuuuuuauuugUGCCgAuuaggccGCGGCGg -3' miRNA: 3'- -GGCguCCGUCUGU--------------ACGGgU-------CGUCGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 4589 | 0.66 | 0.767888 |
|
Target: 5'- aCGCAGGCccuccccGcCAgcGCCgCGGCGGCGUa -3' miRNA: 3'- gGCGUCCGu------CuGUa-CGG-GUCGUCGCA- -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 97464 | 0.66 | 0.7771 |
|
Target: 5'- gUCGCcuGCgAGACucuaCCCGGCGGCGUc -3' miRNA: 3'- -GGCGucCG-UCUGuac-GGGUCGUCGCA- -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 3658 | 0.66 | 0.7771 |
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Target: 5'- gCCGCcgucucgacgauGGGCGGcCGU-CUCGGCGGCGa -3' miRNA: 3'- -GGCG------------UCCGUCuGUAcGGGUCGUCGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 40287 | 0.66 | 0.783475 |
|
Target: 5'- gCCGCgucgcgguucgacgAGGCGGACGgcgaggauccccUGCCCcccgccgcguGCGGCGg -3' miRNA: 3'- -GGCG--------------UCCGUCUGU------------ACGGGu---------CGUCGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 36122 | 0.66 | 0.776185 |
|
Target: 5'- cUCGCcGGCcGACGauuucuuUGCCCAcGUAGCGc -3' miRNA: 3'- -GGCGuCCGuCUGU-------ACGGGU-CGUCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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