Results 101 - 106 of 106 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24398 | 5' | -59.2 | NC_005264.1 | + | 39485 | 0.66 | 0.786188 |
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Target: 5'- gCCGCcagGGGCAGGCcUGCgCuGUGGUGg -3' miRNA: 3'- -GGCG---UCCGUCUGuACGgGuCGUCGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 46412 | 0.66 | 0.794252 |
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Target: 5'- cCCGCGGcCAGACGccCCCAGCGaccugccGCGg -3' miRNA: 3'- -GGCGUCcGUCUGUacGGGUCGU-------CGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 42609 | 0.66 | 0.795141 |
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Target: 5'- cCCGCAGccauggccGCGGAC--GCCCGGUccgcuGCGUa -3' miRNA: 3'- -GGCGUC--------CGUCUGuaCGGGUCGu----CGCA- -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 146777 | 0.66 | 0.795141 |
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Target: 5'- gCCGCGcGGCGGuucUcgGUgCGGCGGUGUg -3' miRNA: 3'- -GGCGU-CCGUCu--GuaCGgGUCGUCGCA- -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 71628 | 0.66 | 0.795141 |
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Target: 5'- gCgGC-GGCAGAgAUaGCCCAGUGcGCGa -3' miRNA: 3'- -GgCGuCCGUCUgUA-CGGGUCGU-CGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 161636 | 0.66 | 0.795141 |
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Target: 5'- cCCGCAGccauggccGCGGAC--GCCCGGUccgcuGCGUa -3' miRNA: 3'- -GGCGUC--------CGUCUGuaCGGGUCGu----CGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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