Results 101 - 106 of 106 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24398 | 5' | -59.2 | NC_005264.1 | + | 26365 | 0.67 | 0.720248 |
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Target: 5'- gCCGC-GGCAGGC-UGCgCGGCGcCGUc -3' miRNA: 3'- -GGCGuCCGUCUGuACGgGUCGUcGCA- -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 38846 | 0.67 | 0.720248 |
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Target: 5'- gCGCGGGCAc-CGUGCCgAGuCGGUGa -3' miRNA: 3'- gGCGUCCGUcuGUACGGgUC-GUCGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 148899 | 0.67 | 0.729957 |
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Target: 5'- gCCGCGuGGCauAGACuUGCUgCGGCAGCu- -3' miRNA: 3'- -GGCGU-CCG--UCUGuACGG-GUCGUCGca -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 124772 | 0.67 | 0.739585 |
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Target: 5'- aCgGCGGGUAGGCAUugugagagcggcGCCUuccacugacaAGCGGCGc -3' miRNA: 3'- -GgCGUCCGUCUGUA------------CGGG----------UCGUCGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 67630 | 0.67 | 0.739585 |
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Target: 5'- aCGCGGGCgcAGGgGUGUCCuGC-GCGg -3' miRNA: 3'- gGCGUCCG--UCUgUACGGGuCGuCGCa -5' |
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| 24398 | 5' | -59.2 | NC_005264.1 | + | 42609 | 0.66 | 0.795141 |
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Target: 5'- cCCGCAGccauggccGCGGAC--GCCCGGUccgcuGCGUa -3' miRNA: 3'- -GGCGUC--------CGUCUGuaCGGGUCGu----CGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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