miRNA: 3'- uuuuCUUAUGCGc---CGGGACG--------------UGGAGC- -5'
miRNA: 3'- uuUUCuuAugCG-CCGGGACgUGGagc -5'
miRNA: 3'- uuUUCUUAUgCGCCGGgACGuGGAGc -5'
miRNA: 3'- -UUUUCUUaug---CGCCGGG---ACGUGGagc -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24400 | 5' | -53.6 | NC_005264.1 | + | 156888 | 0.68 | 0.914732 |
|
Target: 5'- ---cGAAUGCGCcgcuGCCCUGCgcgagggguuccgaGCCUUGa -3' miRNA: 3'- uuuuCUUAUGCGc---CGGGACG--------------UGGAGC- -5' |
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| 24400 | 5' | -53.6 | NC_005264.1 | + | 157353 | 0.67 | 0.93815 |
|
Target: 5'- -cGAGggUugGCgGGCCCUG-GCCg-- -3' miRNA: 3'- uuUUCuuAugCG-CCGGGACgUGGagc -5' |
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| 24400 | 5' | -53.6 | NC_005264.1 | + | 160049 | 0.69 | 0.861475 |
|
Target: 5'- -uGAGGAUGaGCGGCUgUGC-CCUCa -3' miRNA: 3'- uuUUCUUAUgCGCCGGgACGuGGAGc -5' |
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| 24400 | 5' | -53.6 | NC_005264.1 | + | 162736 | 0.66 | 0.955808 |
|
Target: 5'- aAAAGGAAacucgaGCGGCCCgcgUGCGCCg-- -3' miRNA: 3'- -UUUUCUUaug---CGCCGGG---ACGUGGagc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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