Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24402 | 5' | -57.6 | NC_005264.1 | + | 129497 | 0.71 | 0.571557 |
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Target: 5'- gCGGCGGcGCagggUUUGGCGGCGcCGGAGGc -3' miRNA: 3'- -GCUGUC-CGga--GAACCGUCGC-GUCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 131986 | 0.68 | 0.712956 |
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Target: 5'- aCGACAGGCC----GGUGGCGguGGGc -3' miRNA: 3'- -GCUGUCCGGagaaCCGUCGCguCUCu -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 133732 | 0.66 | 0.832671 |
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Target: 5'- cCGACAG-CC-CUU-GCGGCGCuGGGAg -3' miRNA: 3'- -GCUGUCcGGaGAAcCGUCGCGuCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 135575 | 0.69 | 0.672895 |
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Target: 5'- gGGCcaacaGGGCCUCUuccUGGCuacuagagAGCGCAGAa- -3' miRNA: 3'- gCUG-----UCCGGAGA---ACCG--------UCGCGUCUcu -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 142976 | 0.66 | 0.832671 |
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Target: 5'- gCGGC-GGUCUCUcgGGCaugaggcgGGCGUGGGGAc -3' miRNA: 3'- -GCUGuCCGGAGAa-CCG--------UCGCGUCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 156609 | 0.66 | 0.848839 |
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Target: 5'- gCGGCAaGUCUCgcGGCGGCcgcgGCGGGGGg -3' miRNA: 3'- -GCUGUcCGGAGaaCCGUCG----CGUCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 158520 | 0.71 | 0.541661 |
|
Target: 5'- gGGCAGGCCUgCgcugUGGUGGaCGcCAGGGAc -3' miRNA: 3'- gCUGUCCGGA-Ga---ACCGUC-GC-GUCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 159019 | 0.67 | 0.798236 |
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Target: 5'- -aGCAGGUCUCc--GC-GCGCAGGGAg -3' miRNA: 3'- gcUGUCCGGAGaacCGuCGCGUCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 159248 | 0.66 | 0.824313 |
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Target: 5'- gCGGCGGuaauucuucgcGCCUCcUGGCGuuaCGCGGAGGa -3' miRNA: 3'- -GCUGUC-----------CGGAGaACCGUc--GCGUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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