Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24402 | 5' | -57.6 | NC_005264.1 | + | 13936 | 0.66 | 0.824313 |
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Target: 5'- gGAauaGGGUCUU-UGGCuAGCGCgAGAGAu -3' miRNA: 3'- gCUg--UCCGGAGaACCG-UCGCG-UCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 10745 | 0.67 | 0.815783 |
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Target: 5'- cCGAC-GGCaugaUCgaucaggUGGUAGCGCAGAc- -3' miRNA: 3'- -GCUGuCCGg---AGa------ACCGUCGCGUCUcu -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 9929 | 0.67 | 0.807088 |
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Target: 5'- -uGCGGGCUUUgcGGC-GCGCuGAGAg -3' miRNA: 3'- gcUGUCCGGAGaaCCGuCGCGuCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 9627 | 0.68 | 0.728708 |
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Target: 5'- -aGCAGGCCagaUCUggucguuggggggGGCAGUGCAGAu- -3' miRNA: 3'- gcUGUCCGG---AGAa------------CCGUCGCGUCUcu -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 6595 | 0.67 | 0.770827 |
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Target: 5'- cCGACAGGUCaac-GGCgAGCGCGuGGGAa -3' miRNA: 3'- -GCUGUCCGGagaaCCG-UCGCGU-CUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 4433 | 0.67 | 0.813191 |
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Target: 5'- cCGACgucgucaucgucugAGGCCUC-UGGCuGCGCGauucugggcGGGAa -3' miRNA: 3'- -GCUG--------------UCCGGAGaACCGuCGCGU---------CUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 4247 | 0.66 | 0.832671 |
|
Target: 5'- -uGCGGaGCCUCUUGccGCcGCGCAGcGAg -3' miRNA: 3'- gcUGUC-CGGAGAAC--CGuCGCGUCuCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 3675 | 0.67 | 0.807088 |
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Target: 5'- gGGC-GGCCgUCUcGGCGGCGagacgaGGAGGa -3' miRNA: 3'- gCUGuCCGG-AGAaCCGUCGCg-----UCUCU- -5' |
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| 24402 | 5' | -57.6 | NC_005264.1 | + | 2499 | 0.76 | 0.307766 |
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Target: 5'- uCGGCGGGCCUCgcgcgacuuCAGCGCGGGGGg -3' miRNA: 3'- -GCUGUCCGGAGaacc-----GUCGCGUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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