Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24407 | 3' | -59.2 | NC_005264.1 | + | 102782 | 0.69 | 0.617965 |
Target: 5'- -aGUUCGGUGGCCuguguuGCGCGAUGCc--- -3' miRNA: 3'- ccUAGGCCACCGG------CGCGCUGCGaaga -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 107183 | 0.71 | 0.536903 |
Target: 5'- aGGAUaCCGG-GGCCGCcaaaacuaaaggcgGCGGCGCgcgCg -3' miRNA: 3'- -CCUA-GGCCaCCGGCG--------------CGCUGCGaa-Ga -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 108473 | 1.09 | 0.001645 |
Target: 5'- gGGAUCCGGUGGCCGCGCGACGCUUCUa -3' miRNA: 3'- -CCUAGGCCACCGGCGCGCUGCGAAGA- -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 123483 | 0.71 | 0.511261 |
Target: 5'- gGGcAUgCGGUGGCCGCGCGguuGCUgggCg -3' miRNA: 3'- -CC-UAgGCCACCGGCGCGCug-CGAa--Ga -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 124640 | 0.77 | 0.261324 |
Target: 5'- cGGAgCCGcuaucgGGCCGCGCGACGCUa-- -3' miRNA: 3'- -CCUaGGCca----CCGGCGCGCUGCGAaga -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 125207 | 0.67 | 0.781214 |
Target: 5'- uGGGgcacgCCGGUcgccauGGCCGUGCGAgaCGCgUCc -3' miRNA: 3'- -CCUa----GGCCA------CCGGCGCGCU--GCGaAGa -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 146757 | 0.71 | 0.549409 |
Target: 5'- cGGAcCCGGcaacagcaaGcGCCGCGCGGCGgUUCUc -3' miRNA: 3'- -CCUaGGCCa--------C-CGGCGCGCUGCgAAGA- -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 150144 | 0.66 | 0.789206 |
Target: 5'- aGGAUCCGGUcccgggcguGGuCCGCGaCGgacccguGCGgUUCUa -3' miRNA: 3'- -CCUAGGCCA---------CC-GGCGC-GC-------UGCgAAGA- -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 151344 | 0.66 | 0.790087 |
Target: 5'- ----aUGGgcaagGGCCGCGCGGC-CUUCg -3' miRNA: 3'- ccuagGCCa----CCGGCGCGCUGcGAAGa -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 152379 | 0.69 | 0.667351 |
Target: 5'- gGGAUCCGGccuaucGGCgGCGaucCGCUUCg -3' miRNA: 3'- -CCUAGGCCa-----CCGgCGCgcuGCGAAGa -5' |
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24407 | 3' | -59.2 | NC_005264.1 | + | 157472 | 0.66 | 0.819217 |
Target: 5'- cGAgcgCCGGUgugaaucauccgcgaGGCCGCccgggacgaGCGAcCGCUUCg -3' miRNA: 3'- cCUa--GGCCA---------------CCGGCG---------CGCU-GCGAAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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