miRNA: 3'- uGGUGCauaa---CGCGCGACCgc-AUGCG- -5'
miRNA: 3'- ugGUGCa-----UAACGCGCGACCGCau-----GCG- -5'
miRNA: 3'- ugGUGCAUAACG---------------CGCGaCCGCAugCG- -5'
miRNA: 3'- ugGUGCauaaCGCGCGAC-CGCa--UGCG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24415 | 3' | -54.7 | NC_005264.1 | + | 155935 | 0.71 | 0.737102 |
|
Target: 5'- cCCACGgaaagccGCGCGCUGGgacUGCGCg -3' miRNA: 3'- uGGUGCauaa---CGCGCGACCgc-AUGCG- -5' |
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| 24415 | 3' | -54.7 | NC_005264.1 | + | 155990 | 0.74 | 0.554372 |
|
Target: 5'- -gCACGacagagGUUGCGCGCUGGCGccauaguCGCc -3' miRNA: 3'- ugGUGCa-----UAACGCGCGACCGCau-----GCG- -5' |
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| 24415 | 3' | -54.7 | NC_005264.1 | + | 157385 | 0.73 | 0.619548 |
|
Target: 5'- -aCACGUGUUGCugcgcaugcuguacgGCGC-GGCGUugGUa -3' miRNA: 3'- ugGUGCAUAACG---------------CGCGaCCGCAugCG- -5' |
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| 24415 | 3' | -54.7 | NC_005264.1 | + | 162685 | 0.66 | 0.936765 |
|
Target: 5'- -gUACGgucgGCGCGCUGcGCGccgGCGUu -3' miRNA: 3'- ugGUGCauaaCGCGCGAC-CGCa--UGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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