Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24422 | 5' | -58 | NC_005264.1 | + | 91902 | 0.66 | 0.835479 |
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Target: 5'- --cGCGgCagCGaUGGGCGGGcGCGGCGAc -3' miRNA: 3'- guuCGCgGa-GC-ACCUGCUC-CGCUGCU- -5' |
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| 24422 | 5' | -58 | NC_005264.1 | + | 150436 | 0.66 | 0.835479 |
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Target: 5'- gCGGGcCGCCUCG-GGGCGGGaGaucuuauagcuCGACGGg -3' miRNA: 3'- -GUUC-GCGGAGCaCCUGCUC-C-----------GCUGCU- -5' |
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| 24422 | 5' | -58 | NC_005264.1 | + | 34322 | 0.66 | 0.835479 |
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Target: 5'- -uAGCGCCUU-----CGAGGCGGCGGu -3' miRNA: 3'- guUCGCGGAGcaccuGCUCCGCUGCU- -5' |
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| 24422 | 5' | -58 | NC_005264.1 | + | 149630 | 0.67 | 0.827328 |
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Target: 5'- gAAGCaGCC-CG-GcGACGGcGGCGGCGAc -3' miRNA: 3'- gUUCG-CGGaGCaC-CUGCU-CCGCUGCU- -5' |
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| 24422 | 5' | -58 | NC_005264.1 | + | 27900 | 0.67 | 0.827328 |
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Target: 5'- -cAGCGUCUCGgcgaGGGUGAG-CGACGAc -3' miRNA: 3'- guUCGCGGAGCa---CCUGCUCcGCUGCU- -5' |
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| 24422 | 5' | -58 | NC_005264.1 | + | 1006 | 0.67 | 0.827328 |
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Target: 5'- --cGCGCCcgUGUGGugGGGG-GGCa- -3' miRNA: 3'- guuCGCGGa-GCACCugCUCCgCUGcu -5' |
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| 24422 | 5' | -58 | NC_005264.1 | + | 56234 | 0.67 | 0.810524 |
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Target: 5'- gCGAGCGUCgCGcGGcuuuuguCGAGGUGGCGGg -3' miRNA: 3'- -GUUCGCGGaGCaCCu------GCUCCGCUGCU- -5' |
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| 24422 | 5' | -58 | NC_005264.1 | + | 67005 | 0.67 | 0.801887 |
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Target: 5'- gCGAGUGCUgCcUGGACGGaGCGGCGGc -3' miRNA: 3'- -GUUCGCGGaGcACCUGCUcCGCUGCU- -5' |
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| 24422 | 5' | -58 | NC_005264.1 | + | 25926 | 0.82 | 0.134405 |
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Target: 5'- gGGGCGCUguaGcGGACGAGGCGACGAa -3' miRNA: 3'- gUUCGCGGag-CaCCUGCUCCGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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