Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24450 | 5' | -51.7 | NC_005264.1 | + | 121908 | 0.68 | 0.957999 |
Target: 5'- -cGaCCGGggcGCGGCGCGgcGgGGAGGg -3' miRNA: 3'- caC-GGCCau-UGUCGCGCauCaUCUUC- -5' |
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24450 | 5' | -51.7 | NC_005264.1 | + | 20456 | 0.68 | 0.968415 |
Target: 5'- -aGCCGGcggacAGCGGCGCGcaugGGAGGg -3' miRNA: 3'- caCGGCCa----UUGUCGCGCaucaUCUUC- -5' |
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24450 | 5' | -51.7 | NC_005264.1 | + | 68614 | 0.67 | 0.971438 |
Target: 5'- -gGCCGcguagcgcaucGUAGCAaCGCGUGGUAGgcGa -3' miRNA: 3'- caCGGC-----------CAUUGUcGCGCAUCAUCuuC- -5' |
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24450 | 5' | -51.7 | NC_005264.1 | + | 34129 | 0.67 | 0.976849 |
Target: 5'- cUGCCucuGGUGACgGGCGCGUAGg----- -3' miRNA: 3'- cACGG---CCAUUG-UCGCGCAUCaucuuc -5' |
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24450 | 5' | -51.7 | NC_005264.1 | + | 55828 | 0.73 | 0.787108 |
Target: 5'- -aGCCGGUGGCGGCGCGgcugacUGGGc- -3' miRNA: 3'- caCGGCCAUUGUCGCGCauc---AUCUuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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