Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24455 | 5' | -52.5 | NC_005264.1 | + | 8384 | 0.7 | 0.872857 |
Target: 5'- cGGAc---GCGGCGCGUUgaaGCUGCCGg- -3' miRNA: 3'- -CCUuacuCGCUGUGCAA---CGACGGCaa -5' |
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24455 | 5' | -52.5 | NC_005264.1 | + | 127411 | 0.7 | 0.872857 |
Target: 5'- cGGAc---GCGGCGCGUUgaaGCUGCCGg- -3' miRNA: 3'- -CCUuacuCGCUGUGCAA---CGACGGCaa -5' |
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24455 | 5' | -52.5 | NC_005264.1 | + | 52559 | 0.71 | 0.857425 |
Target: 5'- uGGAAUGGGCGGUGCGgauggcacgGCUGCCu-- -3' miRNA: 3'- -CCUUACUCGCUGUGCaa-------CGACGGcaa -5' |
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24455 | 5' | -52.5 | NC_005264.1 | + | 97785 | 0.78 | 0.485865 |
Target: 5'- uGGAUGGGCGGCGCGgcguaaugaguagGCUGCCGa- -3' miRNA: 3'- cCUUACUCGCUGUGCaa-----------CGACGGCaa -5' |
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24455 | 5' | -52.5 | NC_005264.1 | + | 72913 | 1.09 | 0.006224 |
Target: 5'- cGGAAUGAGCGACACGUUGCUGCCGUUg -3' miRNA: 3'- -CCUUACUCGCUGUGCAACGACGGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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