Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24457 | 3' | -55.6 | NC_005264.1 | + | 2665 | 0.68 | 0.858817 |
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Target: 5'- cAGCUcugaCGUCCuaguCGGGUCCGCaAGcgGGCu -3' miRNA: 3'- -UUGA----GCAGGc---GCCUAGGUG-UCuaCCG- -5' |
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| 24457 | 3' | -55.6 | NC_005264.1 | + | 142022 | 0.68 | 0.851021 |
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Target: 5'- cGC-CGUCCGCGcGAUUgGCGuGcAUGGCg -3' miRNA: 3'- uUGaGCAGGCGC-CUAGgUGU-C-UACCG- -5' |
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| 24457 | 3' | -55.6 | NC_005264.1 | + | 142781 | 0.68 | 0.843027 |
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Target: 5'- uGCUCGcuaCGCGGggCCGC--GUGGCg -3' miRNA: 3'- uUGAGCag-GCGCCuaGGUGucUACCG- -5' |
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| 24457 | 3' | -55.6 | NC_005264.1 | + | 61030 | 0.68 | 0.829839 |
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Target: 5'- cGACUCGUCCuaCGGAgccucugagcugucuUCUGCGG-UGGCa -3' miRNA: 3'- -UUGAGCAGGc-GCCU---------------AGGUGUCuACCG- -5' |
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| 24457 | 3' | -55.6 | NC_005264.1 | + | 106851 | 0.69 | 0.817921 |
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Target: 5'- gGACUCGuucaUCUGCGGggUCAUGGGcgGGCa -3' miRNA: 3'- -UUGAGC----AGGCGCCuaGGUGUCUa-CCG- -5' |
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| 24457 | 3' | -55.6 | NC_005264.1 | + | 53271 | 0.69 | 0.800326 |
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Target: 5'- uGGCUUGcugucCCGCGGcgCCGCAGuuUGGUu -3' miRNA: 3'- -UUGAGCa----GGCGCCuaGGUGUCu-ACCG- -5' |
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| 24457 | 3' | -55.6 | NC_005264.1 | + | 140641 | 0.7 | 0.753828 |
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Target: 5'- cACUUG-CCGCGcacgCCACAGAUGGa -3' miRNA: 3'- uUGAGCaGGCGCcua-GGUGUCUACCg -5' |
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| 24457 | 3' | -55.6 | NC_005264.1 | + | 114184 | 0.7 | 0.734413 |
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Target: 5'- aGugUCGgguaacgUCGCGGAUCU--GGAUGGCa -3' miRNA: 3'- -UugAGCa------GGCGCCUAGGugUCUACCG- -5' |
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| 24457 | 3' | -55.6 | NC_005264.1 | + | 70326 | 1.08 | 0.00352 |
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Target: 5'- gAACUCGUCCGCGGAUCCACAGAUGGCc -3' miRNA: 3'- -UUGAGCAGGCGCCUAGGUGUCUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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