Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24459 | 5' | -54.2 | NC_005264.1 | + | 97777 | 0.71 | 0.760208 |
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Target: 5'- -cUCaCGGAUG-GAUGGGCGGcgcggcguaaugaGUAGGCu -3' miRNA: 3'- uaAG-GUCUAUgCUACCCGCC-------------CAUCCG- -5' |
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| 24459 | 5' | -54.2 | NC_005264.1 | + | 91903 | 0.71 | 0.751471 |
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Target: 5'- ---gCGGcaGCGAUGGGCGGGcgcGGCg -3' miRNA: 3'- uaagGUCuaUGCUACCCGCCCau-CCG- -5' |
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| 24459 | 5' | -54.2 | NC_005264.1 | + | 92070 | 0.72 | 0.721756 |
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Target: 5'- -gUCUAGuacgcACGAgGGGCGGGUcauggAGGCg -3' miRNA: 3'- uaAGGUCua---UGCUaCCCGCCCA-----UCCG- -5' |
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| 24459 | 5' | -54.2 | NC_005264.1 | + | 16554 | 0.73 | 0.670777 |
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Target: 5'- uAUUUCAGc-GCGGUGGGCGGcGccgGGGCg -3' miRNA: 3'- -UAAGGUCuaUGCUACCCGCC-Ca--UCCG- -5' |
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| 24459 | 5' | -54.2 | NC_005264.1 | + | 28013 | 0.74 | 0.608618 |
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Target: 5'- -aUCCAGGU-CGGUcggGGGCGGG-AGGUc -3' miRNA: 3'- uaAGGUCUAuGCUA---CCCGCCCaUCCG- -5' |
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| 24459 | 5' | -54.2 | NC_005264.1 | + | 69388 | 1.1 | 0.003422 |
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Target: 5'- uAUUCCAGAUACGAUGGGCGGGUAGGCg -3' miRNA: 3'- -UAAGGUCUAUGCUACCCGCCCAUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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