miRNA: 3'- cGCGG-GUa-CGCUCUGUuuuCUGUUUU- -5'
miRNA: 3'- -CGC-GGGuaCGC-UCUGUUUU--CUGUUuu -5'
miRNA: 3'- -CGCgGGUACGcuCUGUUU--UCUGUuuu -5'
miRNA: 3'- -CGCGGGuaCGCucUGUUUUCUGuuuu -5'
miRNA: 3'- cGCGGGUa----------CGCUCUGUUuuCUGUUUu -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24461 | 5' | -48.4 | NC_005264.1 | + | 157643 | 0.66 | 0.999325 |
|
Target: 5'- uCGCCgCGgcGCGAGACAc--GACAGGAg -3' miRNA: 3'- cGCGG-GUa-CGCUCUGUuuuCUGUUUU- -5' |
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| 24461 | 5' | -48.4 | NC_005264.1 | + | 157839 | 0.7 | 0.98436 |
|
Target: 5'- gGCGaCCCcgGCGcGGACGAGAacGACGAc- -3' miRNA: 3'- -CGC-GGGuaCGC-UCUGUUUU--CUGUUuu -5' |
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| 24461 | 5' | -48.4 | NC_005264.1 | + | 158715 | 0.66 | 0.999325 |
|
Target: 5'- aGCGgCCGUGUuuGGCAGAggGGACAc-- -3' miRNA: 3'- -CGCgGGUACGcuCUGUUU--UCUGUuuu -5' |
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| 24461 | 5' | -48.4 | NC_005264.1 | + | 159090 | 0.72 | 0.940903 |
|
Target: 5'- aGCGCCCcgGCG--GCAAGAGGCc--- -3' miRNA: 3'- -CGCGGGuaCGCucUGUUUUCUGuuuu -5' |
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| 24461 | 5' | -48.4 | NC_005264.1 | + | 159579 | 0.69 | 0.990732 |
|
Target: 5'- aUGCCCAcccaccgagaaGCGAGACGAgcGACGGAc -3' miRNA: 3'- cGCGGGUa----------CGCUCUGUUuuCUGUUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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