Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24467 | 5' | -55.4 | NC_005264.1 | + | 149584 | 0.66 | 0.927494 |
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Target: 5'- --cCCUCGUCGUCCuCCgaGUcgGUAg -3' miRNA: 3'- acaGGAGCGGCAGGuGGgaCAaaCAU- -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 30557 | 0.66 | 0.927494 |
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Target: 5'- --cCCUCGUCGUCCuCCgaGUcgGUAg -3' miRNA: 3'- acaGGAGCGGCAGGuGGgaCAaaCAU- -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 9835 | 0.66 | 0.922042 |
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Target: 5'- -aUCCUCGUCGUCUAUCCaGUUc--- -3' miRNA: 3'- acAGGAGCGGCAGGUGGGaCAAacau -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 62001 | 0.66 | 0.916348 |
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Target: 5'- cGUCgagggagaaCUCGCCGUCguCCCUGUc---- -3' miRNA: 3'- aCAG---------GAGCGGCAGguGGGACAaacau -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 92632 | 0.67 | 0.897825 |
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Target: 5'- aGUCCUUGCCG-CCugCCagcacgaaUGUgcGUAa -3' miRNA: 3'- aCAGGAGCGGCaGGugGG--------ACAaaCAU- -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 136692 | 0.68 | 0.846617 |
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Target: 5'- aGUCCUCGCCGcggCCugggacaucaugGCCCUGg----- -3' miRNA: 3'- aCAGGAGCGGCa--GG------------UGGGACaaacau -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 127107 | 0.69 | 0.794992 |
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Target: 5'- -cUCCUCGCCGgcaCCAUCCUGa----- -3' miRNA: 3'- acAGGAGCGGCa--GGUGGGACaaacau -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 117807 | 0.7 | 0.728143 |
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Target: 5'- -cUCgUCGCCGUCCAUCCUGc----- -3' miRNA: 3'- acAGgAGCGGCAGGUGGGACaaacau -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 3515 | 0.74 | 0.524653 |
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Target: 5'- -aUCCUCGCCGUCCGCCUcGUc---- -3' miRNA: 3'- acAGGAGCGGCAGGUGGGaCAaacau -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 122542 | 0.74 | 0.524653 |
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Target: 5'- -aUCCUCGCCGUCCGCCUcGUc---- -3' miRNA: 3'- acAGGAGCGGCAGGUGGGaCAaacau -5' |
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| 24467 | 5' | -55.4 | NC_005264.1 | + | 60923 | 1.07 | 0.004163 |
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Target: 5'- aUGUCCUCGCCGUCCACCCUGUUUGUAg -3' miRNA: 3'- -ACAGGAGCGGCAGGUGGGACAAACAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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