Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24469 | 5' | -57.3 | NC_005264.1 | + | 86164 | 0.66 | 0.899592 |
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Target: 5'- gCUGGgCAGAGGUaucuucuGcCGcACCGGUGGCc -3' miRNA: 3'- -GGUCaGUCUCCA-------CaGC-UGGCCACCGc -5' |
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| 24469 | 5' | -57.3 | NC_005264.1 | + | 89447 | 0.66 | 0.88734 |
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Target: 5'- -uGGUCAGcGGGUGUUGGCU--UGGCa -3' miRNA: 3'- ggUCAGUC-UCCACAGCUGGccACCGc -5' |
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| 24469 | 5' | -57.3 | NC_005264.1 | + | 162477 | 0.67 | 0.873612 |
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Target: 5'- gCCAGagAGGGGggaaGUCGGgcaaCGGuUGGCGg -3' miRNA: 3'- -GGUCagUCUCCa---CAGCUg---GCC-ACCGC- -5' |
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| 24469 | 5' | -57.3 | NC_005264.1 | + | 43450 | 0.67 | 0.873612 |
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Target: 5'- gCCAGagAGGGGggaaGUCGGgcaaCGGuUGGCGg -3' miRNA: 3'- -GGUCagUCUCCa---CAGCUg---GCC-ACCGC- -5' |
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| 24469 | 5' | -57.3 | NC_005264.1 | + | 129934 | 0.68 | 0.802394 |
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Target: 5'- cCCGGUCAuug--GUCGGCUGGaUGGCGu -3' miRNA: 3'- -GGUCAGUcuccaCAGCUGGCC-ACCGC- -5' |
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| 24469 | 5' | -57.3 | NC_005264.1 | + | 10908 | 0.68 | 0.802394 |
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Target: 5'- cCCGGUCAuug--GUCGGCUGGaUGGCGu -3' miRNA: 3'- -GGUCAGUcuccaCAGCUGGCC-ACCGC- -5' |
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| 24469 | 5' | -57.3 | NC_005264.1 | + | 125839 | 0.77 | 0.342517 |
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Target: 5'- aCGGUCAG-GGUGUUG-CCGGaGGCGg -3' miRNA: 3'- gGUCAGUCuCCACAGCuGGCCaCCGC- -5' |
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| 24469 | 5' | -57.3 | NC_005264.1 | + | 6813 | 0.77 | 0.342517 |
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Target: 5'- aCGGUCAG-GGUGUUG-CCGGaGGCGg -3' miRNA: 3'- gGUCAGUCuCCACAGCuGGCCaCCGC- -5' |
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| 24469 | 5' | -57.3 | NC_005264.1 | + | 59516 | 1.1 | 0.002264 |
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Target: 5'- uCCAGUCAGAGGUGUCGACCGGUGGCGa -3' miRNA: 3'- -GGUCAGUCUCCACAGCUGGCCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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