Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24470 | 5' | -55.4 | NC_005264.1 | + | 815 | 0.67 | 0.887893 |
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Target: 5'- gGGUGCGCGGCCGgcuuuagccUC-UGUCucuggCGuGCCCc -3' miRNA: 3'- aCCAUGUGUCGGU---------AGuACAG-----GC-CGGG- -5' |
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| 24470 | 5' | -55.4 | NC_005264.1 | + | 24061 | 0.67 | 0.887893 |
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Target: 5'- cGG-ACACAGCCAaCAgca-CGGCCa -3' miRNA: 3'- aCCaUGUGUCGGUaGUacagGCCGGg -5' |
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| 24470 | 5' | -55.4 | NC_005264.1 | + | 41770 | 0.68 | 0.873788 |
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Target: 5'- aGGU-CACGGCCuUCAUGaaagCCuGCCUc -3' miRNA: 3'- aCCAuGUGUCGGuAGUACa---GGcCGGG- -5' |
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| 24470 | 5' | -55.4 | NC_005264.1 | + | 147707 | 0.68 | 0.873788 |
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Target: 5'- cGGUGCagACAGaCCAUUccccagagaaagGUGggcCUGGCCCa -3' miRNA: 3'- aCCAUG--UGUC-GGUAG------------UACa--GGCCGGG- -5' |
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| 24470 | 5' | -55.4 | NC_005264.1 | + | 125513 | 0.79 | 0.301132 |
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Target: 5'- gGGUGCGCGGCCAUCGaGggCCGGUagCCg -3' miRNA: 3'- aCCAUGUGUCGGUAGUaCa-GGCCG--GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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