miRNA: 3'- gGCUUGagCUUCCGgaauu---CGUGCAa -5'
miRNA: 3'- gGCUUGAGCuu-CCGGaau-UCGUGcaa -5'
miRNA: 3'- -GGCu-UGAGCU--UCCGGA---AUUCGUGCAa -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24477 | 5' | -50.4 | NC_005264.1 | + | 51291 | 0.66 | 0.996764 |
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Target: 5'- aCGAGCagGAAGGCacccucguGCACGUc -3' miRNA: 3'- gGCUUGagCUUCCGgaauu---CGUGCAa -5' |
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| 24477 | 5' | -50.4 | NC_005264.1 | + | 27645 | 0.66 | 0.996869 |
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Target: 5'- gCGAACUCGuuuGGCCacgcGGCGCu-- -3' miRNA: 3'- gGCUUGAGCuu-CCGGaau-UCGUGcaa -5' |
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| 24477 | 5' | -50.4 | NC_005264.1 | + | 82444 | 0.66 | 0.996869 |
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Target: 5'- uUCGucGCUUGGggGGGCCUcguUGGGUACGUc -3' miRNA: 3'- -GGCu-UGAGCU--UCCGGA---AUUCGUGCAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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