miRNA: 3'- uUAGCGUA-------CGgCGgCAGCGUGC--UCAU- -5'
miRNA: 3'- -UUAGCGua-----CGGCggCAGCGUGCUCau -5'
miRNA: 3'- -UUAGCGuaCGGCGGCAGCG--------------UGCUcau -5'
miRNA: 3'- uuAGCGuACGGCggCAGCgUGCUCAu -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24480 | 3' | -57.1 | NC_005264.1 | + | 149412 | 0.66 | 0.835102 |
|
Target: 5'- cGUCGCGUcucggcgGCgGCgGUCGCGCGccGGUGc -3' miRNA: 3'- uUAGCGUA-------CGgCGgCAGCGUGC--UCAU- -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 160327 | 0.67 | 0.81027 |
|
Target: 5'- aAGUCGCcccccgcGCUGaaGUCGCGCGAGg- -3' miRNA: 3'- -UUAGCGua-----CGGCggCAGCGUGCUCau -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 162199 | 0.66 | 0.841685 |
|
Target: 5'- uAGUCGCcgGUCGCUGUCGUuaucgucgccuccaGCGAa-- -3' miRNA: 3'- -UUAGCGuaCGGCGGCAGCG--------------UGCUcau -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 162253 | 0.68 | 0.735321 |
|
Target: 5'- -cUCGC-UGCCGgaGUCGgGCGAGUc -3' miRNA: 3'- uuAGCGuACGGCggCAGCgUGCUCAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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